DNA barcoding of common Malaysian spiders using the cytochrome oxidase I (COI) gene
For the last twenty years, many newly described spiders were collected from Malaysia and in fact, more than 11,000 species were recorded in Peninsular Malaysia as well as in Sabah and Sarawak states. Scientists have put an immense effort on untangling the spider biology from its physical structure...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Penerbit Universiti Kebangsaan Malaysia
2020
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| Online Access: | http://journalarticle.ukm.my/16803/1/49_03_09.pdf http://journalarticle.ukm.my/16803/ http://www.mabjournal.com/index.php?option=com_content&view=article&id=1039&catid=59:current-view&Itemid=56 |
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| Institution: | Universiti Kebangsaan Malaysia |
| Language: | English |
| Summary: | For the last twenty years, many newly described spiders were collected from Malaysia and in fact, more than 11,000 species
were recorded in Peninsular Malaysia as well as in Sabah and Sarawak states. Scientists have put an immense effort on
untangling the spider biology from its physical structure and behavior to silks and venoms. However, working with spiders
is impeded by the difficulties in species identification via solely morphological methods. Thus, DNA barcoding is an alternative
technique that employs standard fragment to facilitate species identification. Isolation of genomic DNA from three Malaysian
spiders were performed using NucleoSpin® DNA insect extraction kit. Amplification of reference mitochondrial cytochrome
oxidase I (COI) gene employing PCR with two set of primers followed by the DNA sequencing and validation through
phylogenetic analysis were carried out. The commercial extraction kit was effective for the recovery of good quality of intact
genomic DNA band as indicated by the integrity analysis. Both set of primers successfully amplified 100% of the samples
with approximately 600 – 700 bp of PCR products. The obtained sequences (610 bp to 692 bp) were compared with the
sequences available in Gene Bank. BLAST and phylogenetic analysis revealed that the analyzed individual samples belong to
Nephila pilipes, Neoscona nautica and Crossopriza lyoni, respectively. Phylogenetic analysis provided unique insight into
the evolutionary relationship of each analyzed sample. This study aids in an accurate identification of the selected local spider
species at molecular level using the COI gene. |
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