Feasibility of UV-Vis spectral fingerprinting combined with chemometrics for rapid detection of Phyllanthus niruri adulteration with Leucaena leucocephala
Phyllanthus niruri is widely used in Indonesia as immunostimulant. The morphology of Leucaena leucocephala leaves is similar to that of P. niruri leaves. L. leucocephala is easy to find and collect because it is widely distributed in the world. Therefore, it is likely P. niruri could be adultera...
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2021
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my-ukm.journal.171722021-07-26T02:46:19Z http://journalarticle.ukm.my/17172/ Feasibility of UV-Vis spectral fingerprinting combined with chemometrics for rapid detection of Phyllanthus niruri adulteration with Leucaena leucocephala Mohamad Rafi, Nurcahyo, Bayu Wulan Tri Wahyuni, Arif, Zulhan Dewi Anggraini Septaningsih, Sastia Prama Putri, Fukusaki, Eiichiro Phyllanthus niruri is widely used in Indonesia as immunostimulant. The morphology of Leucaena leucocephala leaves is similar to that of P. niruri leaves. L. leucocephala is easy to find and collect because it is widely distributed in the world. Therefore, it is likely P. niruri could be adulterated with L. leucocephala. Therefore, identification and authentication of P. niruri is important to ensure the raw materials used are original without any substitution or mixture with other similar plants causing inconsistencies in their efficacy. In this paper, we described feasibility used of UV-Vis spectral fingerprinting and chemometrics for rapid method for the identification and detection of P. niruri leaves adulterated with L. leucocephala leaves. UV-Vis spectra of samples measured in the interval of 200-800 nm and signal smoothing followed by standard normal variate were used for pre-processing the spectral data. Principal component analysis (PCA) with the absorbance data from the pre-processed UV-Vis spectra in the range of 250-700 nm as variables could distinguish P. niruri from L. leucocephala. PCA followed by discriminant analysis (DA) could successfully classified P. niruri mixed with 5, 25, and 50% L. luecocephala into their respective groups (96.81%). We also employed soft independent modelling of class analogy (SIMCA) for authentication of P. niruri and found that 88.3% of the samples were also correctly classified into their respective groups. A combination of UV-Vis spectroscopy with chemometrics, such as PCA-DA and SIMCA, were used for the first time for the identification and detection of P. niruri adulterated with L. leucocephala. Penerbit Universiti Kebangsaan Malaysia 2021-04 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17172/1/10.pdf Mohamad Rafi, and Nurcahyo, Bayu and Wulan Tri Wahyuni, and Arif, Zulhan and Dewi Anggraini Septaningsih, and Sastia Prama Putri, and Fukusaki, Eiichiro (2021) Feasibility of UV-Vis spectral fingerprinting combined with chemometrics for rapid detection of Phyllanthus niruri adulteration with Leucaena leucocephala. Sains Malaysiana, 50 (4). pp. 997-1006. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid50bil4_2021/KandunganJilid50Bil4_2021.html |
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Phyllanthus niruri is widely used in Indonesia as immunostimulant. The morphology of Leucaena leucocephala leaves
is similar to that of P. niruri leaves. L. leucocephala is easy to find and collect because it is widely distributed in the world.
Therefore, it is likely P. niruri could be adulterated with L. leucocephala. Therefore, identification and authentication
of P. niruri is important to ensure the raw materials used are original without any substitution or mixture with other
similar plants causing inconsistencies in their efficacy. In this paper, we described feasibility used of UV-Vis spectral
fingerprinting and chemometrics for rapid method for the identification and detection of P. niruri leaves adulterated
with L. leucocephala leaves. UV-Vis spectra of samples measured in the interval of 200-800 nm and signal smoothing
followed by standard normal variate were used for pre-processing the spectral data. Principal component analysis (PCA)
with the absorbance data from the pre-processed UV-Vis spectra in the range of 250-700 nm as variables could distinguish
P. niruri from L. leucocephala. PCA followed by discriminant analysis (DA) could successfully classified P. niruri mixed
with 5, 25, and 50% L. luecocephala into their respective groups (96.81%). We also employed soft independent modelling
of class analogy (SIMCA) for authentication of P. niruri and found that 88.3% of the samples were also correctly classified
into their respective groups. A combination of UV-Vis spectroscopy with chemometrics, such as PCA-DA and SIMCA,
were used for the first time for the identification and detection of P. niruri adulterated with L. leucocephala. |
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author |
Mohamad Rafi, Nurcahyo, Bayu Wulan Tri Wahyuni, Arif, Zulhan Dewi Anggraini Septaningsih, Sastia Prama Putri, Fukusaki, Eiichiro |
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Mohamad Rafi, Nurcahyo, Bayu Wulan Tri Wahyuni, Arif, Zulhan Dewi Anggraini Septaningsih, Sastia Prama Putri, Fukusaki, Eiichiro Feasibility of UV-Vis spectral fingerprinting combined with chemometrics for rapid detection of Phyllanthus niruri adulteration with Leucaena leucocephala |
author_facet |
Mohamad Rafi, Nurcahyo, Bayu Wulan Tri Wahyuni, Arif, Zulhan Dewi Anggraini Septaningsih, Sastia Prama Putri, Fukusaki, Eiichiro |
author_sort |
Mohamad Rafi, |
title |
Feasibility of UV-Vis spectral fingerprinting combined with chemometrics for rapid detection of Phyllanthus niruri adulteration with Leucaena leucocephala |
title_short |
Feasibility of UV-Vis spectral fingerprinting combined with chemometrics for rapid detection of Phyllanthus niruri adulteration with Leucaena leucocephala |
title_full |
Feasibility of UV-Vis spectral fingerprinting combined with chemometrics for rapid detection of Phyllanthus niruri adulteration with Leucaena leucocephala |
title_fullStr |
Feasibility of UV-Vis spectral fingerprinting combined with chemometrics for rapid detection of Phyllanthus niruri adulteration with Leucaena leucocephala |
title_full_unstemmed |
Feasibility of UV-Vis spectral fingerprinting combined with chemometrics for rapid detection of Phyllanthus niruri adulteration with Leucaena leucocephala |
title_sort |
feasibility of uv-vis spectral fingerprinting combined with chemometrics for rapid detection of phyllanthus niruri adulteration with leucaena leucocephala |
publisher |
Penerbit Universiti Kebangsaan Malaysia |
publishDate |
2021 |
url |
http://journalarticle.ukm.my/17172/1/10.pdf http://journalarticle.ukm.my/17172/ https://www.ukm.my/jsm/malay_journals/jilid50bil4_2021/KandunganJilid50Bil4_2021.html |
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1706958274312536064 |