The effects of dimmer controlled atmospheric pressure plasma jet to the biophysical structures of HeLa cells
Non-thermal atmospheric pressure plasma jet (NTAPPJ) was shown as a promising tool for sterilization, treatment of chronic wounds & tumors. A portable and facile NTAPPJ system will broaden the application of plasma for many other biomedical applications. This paper demonstrates the feasibility...
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2020
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my-ukm.journal.172172021-07-29T02:50:04Z http://journalarticle.ukm.my/17217/ The effects of dimmer controlled atmospheric pressure plasma jet to the biophysical structures of HeLa cells Chin, Fhong Soon Nafarizal Nayan, Naznin Sultana, Marlia Morsin, Gim, Pao Lim Nyuk, Ling Ma Elfa Rizan Rizon, Kian, Sek Tee Non-thermal atmospheric pressure plasma jet (NTAPPJ) was shown as a promising tool for sterilization, treatment of chronic wounds & tumors. A portable and facile NTAPPJ system will broaden the application of plasma for many other biomedical applications. This paper demonstrates the feasibility of a customized dimmer controllable atmospheric pressure plasma jet (APPJ) in treating HeLa cells. A plasma torch was energized at 8 kVpp and 35 kHz from a neon transformer and discharged with argon gas-producing plasma plume of various gas species. The output intensity of the plasma discharge was adjusted by the voltage input from the dimmer controller circuit. Between 60 – 220 V, a different power output of the dimmer can be varied between 8.4 to 83.6 W, to generate different intensities of non-thermal APPJ (NTAPPJ). The NTAPPJ was then applied to HeLa cells for 20 sec & it was found that cells reduced their adhesion at a dimmer power < 20 W but stayed viable. This progressed to observations of damaged cell body when the power was increased beyond 20 W & the cell membranes shattered when the power was up to 83.6 W. This was due to the ultraviolet rays and reactive oxygen species (ROS), that induced detrimental effects to the cells. The controllable NTAPPJ system, based on dimmer control, induced different levels of effects on the biophysical structures of cells, ranging from reduced cell spread to apoptosis and eventually cell membrane disintegration. Penerbit Universiti Kebangsaan Malaysia 2020 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/17217/1/49_04_02.pdf Chin, Fhong Soon and Nafarizal Nayan, and Naznin Sultana, and Marlia Morsin, and Gim, Pao Lim and Nyuk, Ling Ma and Elfa Rizan Rizon, and Kian, Sek Tee (2020) The effects of dimmer controlled atmospheric pressure plasma jet to the biophysical structures of HeLa cells. Malaysian Applied Biology, 49 (4). pp. 9-17. ISSN 0126-8643 http://www.mabjournal.com/index.php?option=com_content&view=article&id=1070&catid=59:current-view&Itemid=56 |
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Non-thermal atmospheric pressure plasma jet (NTAPPJ) was shown as a promising tool for sterilization, treatment of chronic
wounds & tumors. A portable and facile NTAPPJ system will broaden the application of plasma for many other biomedical
applications. This paper demonstrates the feasibility of a customized dimmer controllable atmospheric pressure plasma jet
(APPJ) in treating HeLa cells. A plasma torch was energized at 8 kVpp and 35 kHz from a neon transformer and discharged
with argon gas-producing plasma plume of various gas species. The output intensity of the plasma discharge was adjusted by
the voltage input from the dimmer controller circuit. Between 60 – 220 V, a different power output of the dimmer can be
varied between 8.4 to 83.6 W, to generate different intensities of non-thermal APPJ (NTAPPJ). The NTAPPJ was then
applied to HeLa cells for 20 sec & it was found that cells reduced their adhesion at a dimmer power < 20 W but stayed
viable. This progressed to observations of damaged cell body when the power was increased beyond 20 W & the cell
membranes shattered when the power was up to 83.6 W. This was due to the ultraviolet rays and reactive oxygen species
(ROS), that induced detrimental effects to the cells. The controllable NTAPPJ system, based on dimmer control, induced
different levels of effects on the biophysical structures of cells, ranging from reduced cell spread to apoptosis and eventually
cell membrane disintegration. |
format |
Article |
author |
Chin, Fhong Soon Nafarizal Nayan, Naznin Sultana, Marlia Morsin, Gim, Pao Lim Nyuk, Ling Ma Elfa Rizan Rizon, Kian, Sek Tee |
spellingShingle |
Chin, Fhong Soon Nafarizal Nayan, Naznin Sultana, Marlia Morsin, Gim, Pao Lim Nyuk, Ling Ma Elfa Rizan Rizon, Kian, Sek Tee The effects of dimmer controlled atmospheric pressure plasma jet to the biophysical structures of HeLa cells |
author_facet |
Chin, Fhong Soon Nafarizal Nayan, Naznin Sultana, Marlia Morsin, Gim, Pao Lim Nyuk, Ling Ma Elfa Rizan Rizon, Kian, Sek Tee |
author_sort |
Chin, Fhong Soon |
title |
The effects of dimmer controlled atmospheric pressure plasma jet to the biophysical structures of HeLa cells |
title_short |
The effects of dimmer controlled atmospheric pressure plasma jet to the biophysical structures of HeLa cells |
title_full |
The effects of dimmer controlled atmospheric pressure plasma jet to the biophysical structures of HeLa cells |
title_fullStr |
The effects of dimmer controlled atmospheric pressure plasma jet to the biophysical structures of HeLa cells |
title_full_unstemmed |
The effects of dimmer controlled atmospheric pressure plasma jet to the biophysical structures of HeLa cells |
title_sort |
effects of dimmer controlled atmospheric pressure plasma jet to the biophysical structures of hela cells |
publisher |
Penerbit Universiti Kebangsaan Malaysia |
publishDate |
2020 |
url |
http://journalarticle.ukm.my/17217/1/49_04_02.pdf http://journalarticle.ukm.my/17217/ http://www.mabjournal.com/index.php?option=com_content&view=article&id=1070&catid=59:current-view&Itemid=56 |
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1706958280535834624 |