Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts

Extracts from Saccharomyces cerevisiae are incorporated in a lot of cosmetic products on the market, but the benefits of the extracts lack scientific reports. We tested the toxicity and anti-senescent activity of an extract from S. cerevisiae on an in vitro model, the human dermal fibroblast (HD...

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Main Authors: Khaizurin Tajul Arifin, Noor Ikhwan Shansuddin, Norwahidah Abdul Karim, Suzana Makpol
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2022
Online Access:http://journalarticle.ukm.my/20452/1/6.pdf
http://journalarticle.ukm.my/20452/
https://www.ukm.my/jsm/malay_journals/jilid51bil8_2022/KandunganJilid51Bil8_2022.html
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Institution: Universiti Kebangsaan Malaysia
Language: English
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spelling my-ukm.journal.204522022-11-10T07:05:15Z http://journalarticle.ukm.my/20452/ Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts Khaizurin Tajul Arifin, Noor Ikhwan Shansuddin, Norwahidah Abdul Karim, Suzana Makpol, Extracts from Saccharomyces cerevisiae are incorporated in a lot of cosmetic products on the market, but the benefits of the extracts lack scientific reports. We tested the toxicity and anti-senescent activity of an extract from S. cerevisiae on an in vitro model, the human dermal fibroblast (HDF) cell culture. We chronicled the development of the extraction method and the subsequent biochemical assays. We used two extraction methods which were hot water extraction and rapid spin. The optimum duration and growth phase to harvest S. cerevisiae were determined by 2,2-diphenyl-1- picrylhydrazyl (DPPH) assay, which also proved that the extracts exhibited antioxidant activity. Hot water extract showed a higher antioxidant activity, and not toxic to HDF. When subjected to senescence-associated β-galactosidase (SA-β-al) assay, the hot water extract significantly reduced the expression of SA-β-Gal in pre-senescent (passage 20, 30 < population doubling < 40) and senescent (passage 30, population doubling > 50) HDF. In conclusion, S. cerevisiae hot water extract possessed antioxidant activity by scavenging DPPH, and anti-senescent activity by reducing the expression of SA-β-Gal in pre-senescent and senescent HDF. Penerbit Universiti Kebangsaan Malaysia 2022-08 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/20452/1/6.pdf Khaizurin Tajul Arifin, and Noor Ikhwan Shansuddin, and Norwahidah Abdul Karim, and Suzana Makpol, (2022) Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts. Sains Malaysiana, 51 (8). pp. 2415-2424. ISSN 0126-6039 https://www.ukm.my/jsm/malay_journals/jilid51bil8_2022/KandunganJilid51Bil8_2022.html
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Extracts from Saccharomyces cerevisiae are incorporated in a lot of cosmetic products on the market, but the benefits of the extracts lack scientific reports. We tested the toxicity and anti-senescent activity of an extract from S. cerevisiae on an in vitro model, the human dermal fibroblast (HDF) cell culture. We chronicled the development of the extraction method and the subsequent biochemical assays. We used two extraction methods which were hot water extraction and rapid spin. The optimum duration and growth phase to harvest S. cerevisiae were determined by 2,2-diphenyl-1- picrylhydrazyl (DPPH) assay, which also proved that the extracts exhibited antioxidant activity. Hot water extract showed a higher antioxidant activity, and not toxic to HDF. When subjected to senescence-associated β-galactosidase (SA-β-al) assay, the hot water extract significantly reduced the expression of SA-β-Gal in pre-senescent (passage 20, 30 < population doubling < 40) and senescent (passage 30, population doubling > 50) HDF. In conclusion, S. cerevisiae hot water extract possessed antioxidant activity by scavenging DPPH, and anti-senescent activity by reducing the expression of SA-β-Gal in pre-senescent and senescent HDF.
format Article
author Khaizurin Tajul Arifin,
Noor Ikhwan Shansuddin,
Norwahidah Abdul Karim,
Suzana Makpol,
spellingShingle Khaizurin Tajul Arifin,
Noor Ikhwan Shansuddin,
Norwahidah Abdul Karim,
Suzana Makpol,
Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts
author_facet Khaizurin Tajul Arifin,
Noor Ikhwan Shansuddin,
Norwahidah Abdul Karim,
Suzana Makpol,
author_sort Khaizurin Tajul Arifin,
title Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts
title_short Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts
title_full Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts
title_fullStr Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts
title_full_unstemmed Hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-Galactosidase (Sa-β-Gal) in human dermal fibroblasts
title_sort hot water extract from saccharomyces cerevisiae scavenges dpph and reduces senescence associated β-galactosidase (sa-β-gal) in human dermal fibroblasts
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2022
url http://journalarticle.ukm.my/20452/1/6.pdf
http://journalarticle.ukm.my/20452/
https://www.ukm.my/jsm/malay_journals/jilid51bil8_2022/KandunganJilid51Bil8_2022.html
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