Expression of BCL-2 and bax genes induced by an ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in breast cancer cell

• Main Authors: Hanif, Monica Mulnia, Djong, Hon Tjong, Syaifullah, Santoso, Putra, Efrizal, Dewi Imelda Roesma
• Format: Article
• Language: English
• Published: Penerbit Universiti Kebangsaan Malaysia 2022
• Online Access: http://journalarticle.ukm.my/20740/1/063-069%2BMAB%2B2283.pdf; http://journalarticle.ukm.my/20740/; https://jms.mabjournal.com/index.php/mab/issue/view/46
• Institution: Universiti Kebangsaan Malaysia

It is known that the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) has the potential for chemoprevention in MCF-7 cells. However, the expression of Bcl-2 and Bax genes was associated with cancer. Therefore, this study aimed to analyze the Bcl-2 and Bax gene expression induced by the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus) in MCF-7. The method in this study was an in vitro experiment using MCF-7 control cells and MCF-7 cells induced by the ovarian extract of Singkarak Lake Pufferfish (Tetraodon leiurus), and Real-time PCR was used to examine the expression of Bcl-2 and Bax genes. The result showed that the expression of the Bcl-2 gene had an insignificant decrease (p>0.05) by 15%, and the expression of the Bax gene had a significant increase (p<0.05) by 489% compared to the control. These results support that ovarian extract Singkarak Lake Pufferfish (Tetraodon leiurus) can be alternative cancer chemoprevention.