Effects of lacticaseibacillus paracasei shirota supplementation on growth performance, intestinal health, and fecal AFB metabolite in AFB1-exposed rats

Aflatoxin B1 (AFB1) is a toxin produced by Aspergillus species of fungi. Findings in the literature has shown the potential of probiotic treatment to alleviate AFB1 toxicity. This study explores the effects of Lacticaseibacillus paracasei Shirota (LcS) supplementation on the growth performance...

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Main Authors: Muhammad Firdhaus Shaharudin, Siti Raihanah Shafie, Mohd Redzwan Sabran, Maizaton Atmadini Abdullah, Fauzah Abd Ghani
Format: Article
Language:English
Published: Penerbit Universiti Kebangsaan Malaysia 2024
Online Access:http://journalarticle.ukm.my/24479/1/Sains%20Kesihatan_22_2_5.pdf
http://journalarticle.ukm.my/24479/
https://ejournals.ukm.my/jskm/issue/view/1646
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Institution: Universiti Kebangsaan Malaysia
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spelling my-ukm.journal.244792024-11-07T00:37:11Z http://journalarticle.ukm.my/24479/ Effects of lacticaseibacillus paracasei shirota supplementation on growth performance, intestinal health, and fecal AFB metabolite in AFB1-exposed rats Muhammad Firdhaus Shaharudin, Siti Raihanah Shafie, Mohd Redzwan Sabran, Maizaton Atmadini Abdullah, Fauzah Abd Ghani, Aflatoxin B1 (AFB1) is a toxin produced by Aspergillus species of fungi. Findings in the literature has shown the potential of probiotic treatment to alleviate AFB1 toxicity. This study explores the effects of Lacticaseibacillus paracasei Shirota (LcS) supplementation on the growth performance, intestinal health, and excretion of faecal AFB1 metabolite of AFB1- exposed rats. Thirty-two male Sprague Dawley rats were divided into control, AFB1, AFB1+LcS and LcS groups. AFB1 was given at a complete dosage of 25 µg AFB1/kg body weight, while LcS supplementation at 2×109 CFU/mL per day for four weeks. The average body weight of the AFB1 group showed no significant increase from week 2 to 4, while other groups had an increment throughout the study. The food intake of the AFB1 and AFB1+LcS groups had significantly reduced throughout the treatment period. AFB1 exposure caused several changes in the histomorphometry parameters but was normalised with LcS supplementation. The AFB1 group showed mild to moderate inflammation in all intestinal parts, whereas only mild inflammation was observed in the jejunum and ileum of the AFB1+LcS group. Faecal Bifidobacterium spp. counts showed an increment in three groups, while the AFB1 group showed a significant reduction. The faecal AFB1 in the AFB1 group was significantly lower than in the AFB1+LcS group. In conclusion, AFB1 affected growth performance and intestinal health, and wherein the effects were alleviated by LcS supplementation. Further investigation on intestinal permeability and serum and urinary AFB1 level is suggested to understand the mechanism of probiotic-AFB1 interaction in alleviating AFB1 toxicity. Penerbit Universiti Kebangsaan Malaysia 2024 Article PeerReviewed application/pdf en http://journalarticle.ukm.my/24479/1/Sains%20Kesihatan_22_2_5.pdf Muhammad Firdhaus Shaharudin, and Siti Raihanah Shafie, and Mohd Redzwan Sabran, and Maizaton Atmadini Abdullah, and Fauzah Abd Ghani, (2024) Effects of lacticaseibacillus paracasei shirota supplementation on growth performance, intestinal health, and fecal AFB metabolite in AFB1-exposed rats. Jurnal Sains Kesihatan Malaysia, 22 (2). pp. 67-82. ISSN 1675-8161 https://ejournals.ukm.my/jskm/issue/view/1646
institution Universiti Kebangsaan Malaysia
building Tun Sri Lanang Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Kebangsaan Malaysia
content_source UKM Journal Article Repository
url_provider http://journalarticle.ukm.my/
language English
description Aflatoxin B1 (AFB1) is a toxin produced by Aspergillus species of fungi. Findings in the literature has shown the potential of probiotic treatment to alleviate AFB1 toxicity. This study explores the effects of Lacticaseibacillus paracasei Shirota (LcS) supplementation on the growth performance, intestinal health, and excretion of faecal AFB1 metabolite of AFB1- exposed rats. Thirty-two male Sprague Dawley rats were divided into control, AFB1, AFB1+LcS and LcS groups. AFB1 was given at a complete dosage of 25 µg AFB1/kg body weight, while LcS supplementation at 2×109 CFU/mL per day for four weeks. The average body weight of the AFB1 group showed no significant increase from week 2 to 4, while other groups had an increment throughout the study. The food intake of the AFB1 and AFB1+LcS groups had significantly reduced throughout the treatment period. AFB1 exposure caused several changes in the histomorphometry parameters but was normalised with LcS supplementation. The AFB1 group showed mild to moderate inflammation in all intestinal parts, whereas only mild inflammation was observed in the jejunum and ileum of the AFB1+LcS group. Faecal Bifidobacterium spp. counts showed an increment in three groups, while the AFB1 group showed a significant reduction. The faecal AFB1 in the AFB1 group was significantly lower than in the AFB1+LcS group. In conclusion, AFB1 affected growth performance and intestinal health, and wherein the effects were alleviated by LcS supplementation. Further investigation on intestinal permeability and serum and urinary AFB1 level is suggested to understand the mechanism of probiotic-AFB1 interaction in alleviating AFB1 toxicity.
format Article
author Muhammad Firdhaus Shaharudin,
Siti Raihanah Shafie,
Mohd Redzwan Sabran,
Maizaton Atmadini Abdullah,
Fauzah Abd Ghani,
spellingShingle Muhammad Firdhaus Shaharudin,
Siti Raihanah Shafie,
Mohd Redzwan Sabran,
Maizaton Atmadini Abdullah,
Fauzah Abd Ghani,
Effects of lacticaseibacillus paracasei shirota supplementation on growth performance, intestinal health, and fecal AFB metabolite in AFB1-exposed rats
author_facet Muhammad Firdhaus Shaharudin,
Siti Raihanah Shafie,
Mohd Redzwan Sabran,
Maizaton Atmadini Abdullah,
Fauzah Abd Ghani,
author_sort Muhammad Firdhaus Shaharudin,
title Effects of lacticaseibacillus paracasei shirota supplementation on growth performance, intestinal health, and fecal AFB metabolite in AFB1-exposed rats
title_short Effects of lacticaseibacillus paracasei shirota supplementation on growth performance, intestinal health, and fecal AFB metabolite in AFB1-exposed rats
title_full Effects of lacticaseibacillus paracasei shirota supplementation on growth performance, intestinal health, and fecal AFB metabolite in AFB1-exposed rats
title_fullStr Effects of lacticaseibacillus paracasei shirota supplementation on growth performance, intestinal health, and fecal AFB metabolite in AFB1-exposed rats
title_full_unstemmed Effects of lacticaseibacillus paracasei shirota supplementation on growth performance, intestinal health, and fecal AFB metabolite in AFB1-exposed rats
title_sort effects of lacticaseibacillus paracasei shirota supplementation on growth performance, intestinal health, and fecal afb metabolite in afb1-exposed rats
publisher Penerbit Universiti Kebangsaan Malaysia
publishDate 2024
url http://journalarticle.ukm.my/24479/1/Sains%20Kesihatan_22_2_5.pdf
http://journalarticle.ukm.my/24479/
https://ejournals.ukm.my/jskm/issue/view/1646
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