Development and validation of gas chromatography-mass spectrometric method for the determination of epoxidized soybean oil in foods stored in glass jars with metal lids

• Main Authors: Hong, Keat Quek, Mohd Marsin Sanagi, Wan Aini Wan Ibrahim, Syairah Salleh, Mazidatulakmam Miskam
• Format: Article
• Language: English
• Published: Universiti Kebangsaan Malaysia 2014
• Online Access: http://journalarticle.ukm.my/6926/1/10_Hong_Keat_Quek.pdf; http://journalarticle.ukm.my/6926/; http://www.ukm.my/jsm/
• Institution: Universiti Kebangsaan Malaysia

A method has been developed for the determination of epoxidized soybean oil (ESBO) in oily foods stored in glass jars with metal lids using gas chromatography-mass spectrometry (GC-MS). ESBO and its internal standard (cis,cis-11,12;14,15-diepoxyeicosanoate) were isolated from the matrix by transesterification process. The developed method showed good linear dynamic range between 0.7-20 μg mL-1 with coefficients of determination (R2) > 0.9968 and acceptable limit of detection and limit of quantitation of 7 and 23 mg kg-1, respectively, based on linearity calculations. Analyte recoveries were 90.84±27.24% for low concentration, 78.05±11.59% for medium concentration and 99.23±10.20% for high concentration. This first fully validated GC-MS method was successfully applied for the determination of ESBO in foods stored in glass jar with metal lid. Among the 31 food samples studied, 6 samples were found to exceed the specific migration limit of 60 mg kg-1 (based on EU Directive 2002/72/EC). The developed method is thus potentially useful for routine analysis for the determination of ESBO.