Copy Number Status of TERT Gene in a Myeloid Leukaemia Cell Line, K562: Application of Interphase Fluorescence in situ Hybridization (FISH) Analysis

• Main Authors: Ahmad Syibli, Othman, Nor Atifah, Mohd Adam, Ravindran, Ankathil, Rosline, Hassan
• Format: Article
• Language: English
• Published: 2011
• Subjects: Q Science (General)
• Online Access: http://eprints.unisza.edu.my/2053/1/FH02-FSK-19-25054.pdf; http://eprints.unisza.edu.my/2053/
• Institution: Universiti Sultan Zainal Abidin

Telomeraseisribonucleoproteinenzymecomplexconsistingoftwomaincorecomponents;reversetranscriptase(TERT),andRNAtemplatefortelomericDNAsynthesis(TERC).TERTgeneactsascatalyticcomponentinthisenzymeandislocatedonchromosome5p15.IthasbeenreportedthatthereisanamplificationorincreaseofTERTgenecopynumberinhumancancercelllinesandprimarytumoursampleswhichmayassociatewithincreasedtelomeraseactivity.Inourstudy,dual-colorassayprobewasusedinfluorescencein situhybridization(FISH)analysisthatcoveredthegenomicregionencodingTERT(Redsignal)togetherwithaspecificsequenceofEGR1(Greensignal)at5q31tofacilitatechromosomeidentification.Innormalcells,theFISHsignalpatternwasobservedastwogreensignalsandtworedsignals(2G2R).AmplificationoftheTERTgenehasbeendetectedinmorethan90%ofthecellsincelllineintheleukaemia.Besidesthat,aneusomyofchromosome5wasalsodetectedindicatingthepresenceofthreecopiesofchromosome5with3G3Rsignalpattern.Thus,theapplicationofinterphaseFISHtechniquefordetectionofTERTgeneamplificationhasbeensuccessfullyoptimized