In Silico Sequence Analysis of Coagulase in Staphylococcus aureus
Coagulase production is generally accepted as being characteristic of pathogenic and potentially pathogenic strains. Coagulase can cause clot formation in the immediate vicinity of the bacterium. The protein has been shown to contribute to bacterial virulence in wound infections owing to its a...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/3771/1/FH02-FBIM-19-25214.pdf http://eprints.unisza.edu.my/3771/ |
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| Institution: | Universiti Sultan Zainal Abidin |
| Language: | English |
| Summary: | Coagulase production is generally accepted as being characteristic of pathogenic
and potentially pathogenic strains. Coagulase can cause clot formation in the
immediate vicinity of the bacterium. The protein has been shown to contribute to
bacterial virulence in wound infections owing to its ability to delay the healing
processes. In this study, we conducted in silico sequence analysis using coagulase
from the Gram-positive human pathogen Staphylococcus aureus with GenBank
Accession no. CAC 84776.1. Various bioinformatics tools were used to predict the
properties of the S. aureus coagulase. The N-terminal portion of coagulase was
predicted to form transmembrane helices whereas the majority of the protein was
hydrophilic in nature and was predicted to form several alpha-helices. Coagulase
was also predicted to localize extracellularly and has a pI value of 8.41. The in silico
analysis carried out offers an alternative way to obtain structural information and
will assist in determining the structural prediction of the S. aureus coagulase. |
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