Microstructural changes in the thyroid gland of the preweaning and weaning rats after heterotypic stress
Chronic stress modulates the activity of various neuroendocrine axes; while activation pattern of the hypothalamo-hypophyseo-adrenal axis (HHAA) is studied in details, the response of the hypothalamo-hypophyseo-thyroid axis (HHTA) is less understood, especially in terms of its response to differen...
Saved in:
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
2019
|
Subjects: | |
Online Access: | http://eprints.unisza.edu.my/6457/1/FH02-FP-19-29959.pdf http://eprints.unisza.edu.my/6457/ |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Universiti Sultan Zainal Abidin |
Language: | English |
id |
my-unisza-ir.6457 |
---|---|
record_format |
eprints |
institution |
Universiti Sultan Zainal Abidin |
building |
UNISZA Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
Universiti Sultan Zainal Abidin |
content_source |
UNISZA Institutional Repository |
url_provider |
https://eprints.unisza.edu.my/ |
language |
English |
topic |
QH301 Biology R Medicine (General) |
spellingShingle |
QH301 Biology R Medicine (General) Gupalo, Sergey Marina, Kapitonova Azhar, Ahmad Syed Baharom, Syed Ahmad Fuad Microstructural changes in the thyroid gland of the preweaning and weaning rats after heterotypic stress |
description |
Chronic stress modulates the activity of various neuroendocrine axes; while activation pattern of the hypothalamo-hypophyseo-adrenal
axis (HHAA) is studied in details, the response of the hypothalamo-hypophyseo-thyroid axis (HHTA) is less understood, especially in
terms of its response to different types of stressors by the follicular and parafollicular compartments of the thyroid gland. Recent papers
presented data on the inhibitory effect of chronic stress on the HHTA, while exercise and low temperature were shown to be able to
activate its function. Most studies were done on the adult experimental animals using genetic studies without consideration of the
structural changes in the peripheral link of the HHTA in the growing body. Reports on the effect of the chronic variable stressors on the
thyroid gland in early life are scarce, though during this period neuroendocrine axes are extremely sensitive to different adverse factors,
such as stress, infection, inflammation and environmental changes. The objective of this research is to assess the microscopic changes in
the thyroid gland of the preweaning and weaning rats exposed to chronic heterotypic stress compared to the homotypic one. Homo- or
heterotypic stressors were chronically applied to the preweaning and weaning rat pups. After the end of the last stress session the
animals were euthanized, thyroid gland was sampled, embedded in paraffin, sectioned and stained for thyroglobulin, calcitonin,
proliferative cells nuclear antigen (PCNA) and caspase 3. The mucosa of the alimentary tract of the experimental animals was
examined and the thymus and the adrenal glands were sampled and weighed to evaluate the depth of stress-induced changes in the body.
Immunologically stained slides of the thyroid gland were assessed using Image Pro+ software. Our study showed that chronic stress
resulted in the structural and immunohistochemical changes of the thyroid gland in the preweaning and weaning experimental animals
which indicate an inhibition of its function in the type of stress-related pattern. Both homo- and heterotypic stressors caused microscopic
alterations in the thyroid gland, the extent of which depended both on the initial age of the experimental animal and the type of the
stressor applied. The number and the size of the thyroglobulin-positive cells significantly decreased, and the volume density of the
apoptotic cells significantly increased in the heterotypically stressed rat pups of both age subgroups with higher level of significance in
the preweaning age subgroup. The number of calcitoninocytes was significantly increased in the weaning rat pups exposed to the
heterotypic stress. The number of PCNA-positive cells significantly decreased only in the heterotypic stress group of both ages with
higher level of significance in the preweaning age subgroup. A positive correlation was found between the volume density of the
thyroglobulin-positive cells and the severity of the accidental thymic involution. Thus, our research demonstrated that weaning period is
very sensitive to chronic stress for the thyroid gland of the experimental animals which by this age becomes mature enough to
differentially respond to the various types of stress (homotypic versus heterotypic) both by the thyroid follicular and parafollicular
compartments, and that thymus plays an important role in the functional capacity of the thyroid gland during stress in early life. |
format |
Article |
author |
Gupalo, Sergey Marina, Kapitonova Azhar, Ahmad Syed Baharom, Syed Ahmad Fuad |
author_facet |
Gupalo, Sergey Marina, Kapitonova Azhar, Ahmad Syed Baharom, Syed Ahmad Fuad |
author_sort |
Gupalo, Sergey |
title |
Microstructural changes in the thyroid gland of the preweaning and weaning rats after heterotypic stress |
title_short |
Microstructural changes in the thyroid gland of the preweaning and weaning rats after heterotypic stress |
title_full |
Microstructural changes in the thyroid gland of the preweaning and weaning rats after heterotypic stress |
title_fullStr |
Microstructural changes in the thyroid gland of the preweaning and weaning rats after heterotypic stress |
title_full_unstemmed |
Microstructural changes in the thyroid gland of the preweaning and weaning rats after heterotypic stress |
title_sort |
microstructural changes in the thyroid gland of the preweaning and weaning rats after heterotypic stress |
publishDate |
2019 |
url |
http://eprints.unisza.edu.my/6457/1/FH02-FP-19-29959.pdf http://eprints.unisza.edu.my/6457/ |
_version_ |
1728056367922544640 |
spelling |
my-unisza-ir.64572022-03-21T02:10:18Z http://eprints.unisza.edu.my/6457/ Microstructural changes in the thyroid gland of the preweaning and weaning rats after heterotypic stress Gupalo, Sergey Marina, Kapitonova Azhar, Ahmad Syed Baharom, Syed Ahmad Fuad QH301 Biology R Medicine (General) Chronic stress modulates the activity of various neuroendocrine axes; while activation pattern of the hypothalamo-hypophyseo-adrenal axis (HHAA) is studied in details, the response of the hypothalamo-hypophyseo-thyroid axis (HHTA) is less understood, especially in terms of its response to different types of stressors by the follicular and parafollicular compartments of the thyroid gland. Recent papers presented data on the inhibitory effect of chronic stress on the HHTA, while exercise and low temperature were shown to be able to activate its function. Most studies were done on the adult experimental animals using genetic studies without consideration of the structural changes in the peripheral link of the HHTA in the growing body. Reports on the effect of the chronic variable stressors on the thyroid gland in early life are scarce, though during this period neuroendocrine axes are extremely sensitive to different adverse factors, such as stress, infection, inflammation and environmental changes. The objective of this research is to assess the microscopic changes in the thyroid gland of the preweaning and weaning rats exposed to chronic heterotypic stress compared to the homotypic one. Homo- or heterotypic stressors were chronically applied to the preweaning and weaning rat pups. After the end of the last stress session the animals were euthanized, thyroid gland was sampled, embedded in paraffin, sectioned and stained for thyroglobulin, calcitonin, proliferative cells nuclear antigen (PCNA) and caspase 3. The mucosa of the alimentary tract of the experimental animals was examined and the thymus and the adrenal glands were sampled and weighed to evaluate the depth of stress-induced changes in the body. Immunologically stained slides of the thyroid gland were assessed using Image Pro+ software. Our study showed that chronic stress resulted in the structural and immunohistochemical changes of the thyroid gland in the preweaning and weaning experimental animals which indicate an inhibition of its function in the type of stress-related pattern. Both homo- and heterotypic stressors caused microscopic alterations in the thyroid gland, the extent of which depended both on the initial age of the experimental animal and the type of the stressor applied. The number and the size of the thyroglobulin-positive cells significantly decreased, and the volume density of the apoptotic cells significantly increased in the heterotypically stressed rat pups of both age subgroups with higher level of significance in the preweaning age subgroup. The number of calcitoninocytes was significantly increased in the weaning rat pups exposed to the heterotypic stress. The number of PCNA-positive cells significantly decreased only in the heterotypic stress group of both ages with higher level of significance in the preweaning age subgroup. A positive correlation was found between the volume density of the thyroglobulin-positive cells and the severity of the accidental thymic involution. Thus, our research demonstrated that weaning period is very sensitive to chronic stress for the thyroid gland of the experimental animals which by this age becomes mature enough to differentially respond to the various types of stress (homotypic versus heterotypic) both by the thyroid follicular and parafollicular compartments, and that thymus plays an important role in the functional capacity of the thyroid gland during stress in early life. 2019-11 Article PeerReviewed text en http://eprints.unisza.edu.my/6457/1/FH02-FP-19-29959.pdf Gupalo, Sergey and Marina, Kapitonova and Azhar, Ahmad and Syed Baharom, Syed Ahmad Fuad (2019) Microstructural changes in the thyroid gland of the preweaning and weaning rats after heterotypic stress. Indo American Journal of Pharmaceutical Sciences, 6 (8). pp. 12071-12082. ISSN 2349-7750 |