The AC/AG Diplotype for the 118A>G and IVS2 + 691G>C Polymorphisms of OPRM1 Gene is Associated with Sleep Quality Among OpioidDependent Patients on Methadone Maintenance Therapy
Introduction Methadone is a full agonist of the opioid receptor mu 1 which is encoded by the OPRM1 gene. Sleep disorders were frequently reported by opioid-dependent patients during methadone maintenance therapy (MMT). It is possible, therefore, that genetic polymorphisms in OPRM1 influence sleep qu...
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my-unisza-ir.71952022-09-13T04:23:49Z http://eprints.unisza.edu.my/7195/ The AC/AG Diplotype for the 118A>G and IVS2 + 691G>C Polymorphisms of OPRM1 Gene is Associated with Sleep Quality Among OpioidDependent Patients on Methadone Maintenance Therapy Nasir, Mohamad Zalina, Zahari Nurfadhlina, Musa QH301 Biology Introduction Methadone is a full agonist of the opioid receptor mu 1 which is encoded by the OPRM1 gene. Sleep disorders were frequently reported by opioid-dependent patients during methadone maintenance therapy (MMT). It is possible, therefore, that genetic polymorphisms in OPRM1 influence sleep quality among patients on MMT. This study investigated the association of OPRM1 polymorphisms with sleep quality among opioid-dependent patients on MMT. Methods The sleep quality of 165 male opioid-dependent patients receiving MMT was evaluated using the Pittsburgh Sleep Quality Index (PSQI). DNA was extracted from whole blood and subjected to polymerase chain reaction (PCR) genotyping. Results Patients with IVS2 + 691 CC genotype had higher PSQI scores [mean (SD) = 5.73 (2.89)] compared to those without the IVS2 + 691 CC genotype (IVS2 + 691 GG/GC genotype) [4.92 (2.31)], but the difference did not reach statistical significance (p = 0.081). Patients with combined 118 AA genotype and IVS2 + 691 GC genotype (AC/AG diplotype) had significantly lower PSQI scores [mean (SD) = 4.25 (2.27)] compared to those without the diplotype [5.68 (2.77)] (p = 0.018). Conclusion Our study indicates that the AC/AG diplotype for the 118A>G and IVS2 + 691G>C polymorphisms of OPRM1 gene is associated with better sleep quality among males with opioid dependence on MMT. 2016 Article PeerReviewed text en http://eprints.unisza.edu.my/7195/1/FH02-FP-16-05478.pdf image en http://eprints.unisza.edu.my/7195/2/FH02-FP-16-06929.jpg Nasir, Mohamad and Zalina, Zahari and Nurfadhlina, Musa (2016) The AC/AG Diplotype for the 118A>G and IVS2 + 691G>C Polymorphisms of OPRM1 Gene is Associated with Sleep Quality Among OpioidDependent Patients on Methadone Maintenance Therapy. Pain and Therapy, 5 (1). ISSN 2193-651X |
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QH301 Biology Nasir, Mohamad Zalina, Zahari Nurfadhlina, Musa The AC/AG Diplotype for the 118A>G and IVS2 + 691G>C Polymorphisms of OPRM1 Gene is Associated with Sleep Quality Among OpioidDependent Patients on Methadone Maintenance Therapy |
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Introduction Methadone is a full agonist of the opioid receptor mu 1 which is encoded by the OPRM1 gene. Sleep disorders were frequently reported by opioid-dependent patients during methadone maintenance therapy (MMT). It is possible, therefore, that genetic polymorphisms in OPRM1 influence sleep quality among patients on MMT. This study investigated the association of OPRM1 polymorphisms with sleep quality among opioid-dependent patients on MMT.
Methods The sleep quality of 165 male opioid-dependent patients receiving MMT was evaluated using the Pittsburgh Sleep Quality Index (PSQI). DNA was extracted from whole blood and subjected to polymerase chain reaction (PCR) genotyping.
Results Patients with IVS2 + 691 CC genotype had higher PSQI scores [mean (SD) = 5.73 (2.89)] compared to those without the IVS2 + 691 CC genotype (IVS2 + 691 GG/GC genotype) [4.92 (2.31)], but the difference did not reach statistical significance (p = 0.081). Patients with combined 118 AA genotype and IVS2 + 691 GC genotype (AC/AG diplotype) had significantly lower PSQI scores [mean (SD) = 4.25 (2.27)] compared to those without the diplotype [5.68 (2.77)] (p = 0.018).
Conclusion Our study indicates that the AC/AG diplotype for the 118A>G and IVS2 + 691G>C polymorphisms of OPRM1 gene is associated with better sleep quality among males with opioid dependence on MMT. |
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Article |
author |
Nasir, Mohamad Zalina, Zahari Nurfadhlina, Musa |
author_facet |
Nasir, Mohamad Zalina, Zahari Nurfadhlina, Musa |
author_sort |
Nasir, Mohamad |
title |
The AC/AG Diplotype for the 118A>G and IVS2 + 691G>C Polymorphisms of OPRM1 Gene is Associated with Sleep Quality Among OpioidDependent Patients on Methadone Maintenance Therapy |
title_short |
The AC/AG Diplotype for the 118A>G and IVS2 + 691G>C Polymorphisms of OPRM1 Gene is Associated with Sleep Quality Among OpioidDependent Patients on Methadone Maintenance Therapy |
title_full |
The AC/AG Diplotype for the 118A>G and IVS2 + 691G>C Polymorphisms of OPRM1 Gene is Associated with Sleep Quality Among OpioidDependent Patients on Methadone Maintenance Therapy |
title_fullStr |
The AC/AG Diplotype for the 118A>G and IVS2 + 691G>C Polymorphisms of OPRM1 Gene is Associated with Sleep Quality Among OpioidDependent Patients on Methadone Maintenance Therapy |
title_full_unstemmed |
The AC/AG Diplotype for the 118A>G and IVS2 + 691G>C Polymorphisms of OPRM1 Gene is Associated with Sleep Quality Among OpioidDependent Patients on Methadone Maintenance Therapy |
title_sort |
ac/ag diplotype for the 118a>g and ivs2 + 691g>c polymorphisms of oprm1 gene is associated with sleep quality among opioiddependent patients on methadone maintenance therapy |
publishDate |
2016 |
url |
http://eprints.unisza.edu.my/7195/1/FH02-FP-16-05478.pdf http://eprints.unisza.edu.my/7195/2/FH02-FP-16-06929.jpg http://eprints.unisza.edu.my/7195/ |
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