Application of PCR-RFLP Technique in the Analysis of MYO1H Single Nucleotide Polymorphism in Malay Mandibular Prognathism Patients
Genetic studies have reported the association between polymorphism in MYO1H with mandibular prognathism. MYO1H is found in skeletal muscle sarcomeres and is expressed in the mandibular jaw cartilage signifying its importance during craniofacial development. This study aimed to characterise the ge...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
2020
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| Subjects: | |
| Online Access: | http://eprints.unisza.edu.my/7552/1/FH02-FSK-20-49476.pdf http://eprints.unisza.edu.my/7552/ |
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| Institution: | Universiti Sultan Zainal Abidin |
| Language: | English |
| Summary: | Genetic studies have reported the association between polymorphism in MYO1H with mandibular
prognathism. MYO1H is found in skeletal muscle sarcomeres and is expressed in the mandibular jaw
cartilage signifying its importance during craniofacial development. This study aimed to characterise
the genotype and allele of MYO1H single nucleotide polymorphism (SNP) (rs3825393) and to
associate the SNP with mandibular prognathism in Class III skeletal malocclusion. This was a casecontrol study, which involved 57 Malay subjects with 30 Class I (control) and 27 Class III skeletal
base patients (case). Cephalometric measurements were taken prior to collection of saliva samples.
MYO1H SNP (rs3825383) was genotyped using polymerase chain reaction-restriction fragment length
polymorphism (PCR-RFLP). Chi-square (χ2
) test was used to compare genotype and allele frequencies
between the groups while Hardy-Weinberg Equilibrium (HWE) was applied to assess distribution of
genotype frequency in both classes. MYO1H SNP (rs3825393) did not yield significant association
with mandibular prognathism with p = 0.33; OR = 0.66; 95% CI = 0.289~1.518, that was reflected
by no significant difference in allele (p > 0.05) and genotype (p > 0.05) frequency between control and
study group. Nevertheless, AA genotype depicted the highest frequency in both groups. The genotype
distribution in both groups was in concordance with HWE (p > 0.05). Our data showed no association
of MYO1H SNP (rs3825393) with mandibular prognathism. Interestingly, we observed Allele A
representing the major allele in Malay population. Presence of MYO1H SNP (rs3825393) was detected
in samples analysed. Larger number of samples is required to confirm the involvement of MYO1H
polymorphisms in mandibular prognathism. |
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