Total flavonoid content, total phenolic content, and the antioxidant activity of Hurrem Titisemas Liquid

• Main Authors: Ismail, Azlini, Tuan Anuar, Tuan Ashraf Faiz, Mustafa, Basma Ezzat, Lestari, Widya, Darnis, Deny Susanti
• Format: Conference or Workshop Item
• Language: English
• Published: 2022
• Subjects: RM Therapeutics. Pharmacology; RM300 Drugs and their action; RS Pharmacy and materia medica
• Online Access: http://irep.iium.edu.my/100433/1/100433_Total%20flavonoid%20content%2C%20total%20phenolic%20content%2C%20and%20the%20antioxidant.pdf; http://irep.iium.edu.my/100433/
• Institution: Universiti Islam Antarabangsa Malaysia

Antioxidants are widely present in a range of foods and medicinal plants, and they play a pertinent role in the prevention and treatment of chronic illnesses induced by oxidative stress such as cancer, cardiovascular diseases, diabetes, obesity, neurodegenerative diseases, as well as in the aging process. HURREM TITISEMAS LIQUID (HTL) is a product registered with Ministry of Health, Malaysia (MAL21106122TC) under natural product-traditional (general) category. This study aims to identify the Total Flavonoid Content (TFC), the Total Phenolic Content (TPC) and the antioxidant activity of HTL. The antioxidant activity of HTL was investigated in three replicates (n=3) using the following antioxidant assays: 1) 2,2-diphenyl-1-picrylhydrazyl (DPPH); 2) 2,20 -azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS); and 3) Ferric-Reducing Antioxidant Power (FRAP) assays. Trolox, ascorbic acid and quercetin were used as standards in these assays respectively. This study found that HTL has DPPH scavenging activity of 62.18 ± 0.96 %. When tested using ABTS assay, HTL showed 82.55 ± 1.01 % of antioxidant capacity and it has ferric reducing power of 2,096.00 ± 0.86 mM Fe2+/g dry mass when analysed using FRAP assay. HTL was also found to contain TFC of 9,993.75 ± 85.54 mg quercetin equivalents/g and the TPC of 486.50 ± 12.48 mg gallic acid equivalents/g. In summary, HTL has antioxidant activity which was presumably related to its phenolics and flavonoids contents.