Mitophagy: a bridge linking HMGB1 and Parkinson’s disease using adult zebrafish as a model organism
High-mobility group box 1 (HMGB1) has been implicated as a key player in two criticalfactors of Parkinson’s disease (PD): mitochondrial dysfunction and neuroinflammation. However, thespecific role of HMGB1 in PD remains elusive. We investigated the effect of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyrid...
Saved in:
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | English English |
Published: |
Multidisciplinary Digital Publishing Institute (MDPI)
2023
|
Subjects: | |
Online Access: | http://irep.iium.edu.my/105633/7/105633_Mitophagy%20a%20bridge%20linking%20HMGB1%20and%20Parkinson%E2%80%99s%20disease.pdf http://irep.iium.edu.my/105633/13/105633_Mitophagy%20a%20bridge%20linking%20HMGB1%20and%20Parkinson%E2%80%99s%20disease_Scopus.pdf http://irep.iium.edu.my/105633/ https://www.mdpi.com/2076-3425/13/7/1076/pdf?version=1689487671 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Universiti Islam Antarabangsa Malaysia |
Language: | English English |
id |
my.iium.irep.105633 |
---|---|
record_format |
dspace |
spelling |
my.iium.irep.1056332024-05-21T08:33:23Z http://irep.iium.edu.my/105633/ Mitophagy: a bridge linking HMGB1 and Parkinson’s disease using adult zebrafish as a model organism Razali, Khairiah Mohd Nasir, Mohd Hamzah Kumar, Jaya Mohamed, Wael Mohamed Yousef R Medicine (General) High-mobility group box 1 (HMGB1) has been implicated as a key player in two criticalfactors of Parkinson’s disease (PD): mitochondrial dysfunction and neuroinflammation. However, thespecific role of HMGB1 in PD remains elusive. We investigated the effect of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administration on mitochondrial dysfunction and HMGB1-associatedinflammatory genes as well as locomotor activity in zebrafish, aiming to elucidate the role of HMGB1in PD. Adult zebrafish received MPTP injections, and locomotor activity was measured at 24- and48-h post-administration. Gene expression levels related to mitophagy (fis1, pink1, and park2) andHMGB1-mediated inflammation (hmgb1, tlr4, and nfkb) were quantified through RT-qPCR analysis.Following MPTP injection, the significant increase in transcript levels of fis1, pink1, and park2 in-dicated notable changes in PINK1/Parkin mitophagy, while the upregulation of hmgb1, tlr4, andnfkb genes pointed to the activation of the HMGB1/TLR4/NFκB inflammatory pathway. Further-more, MPTP-injected zebrafish exhibited decreased locomotor activity, evident through reduceddistance travelled, mean speed, and increased freezing durations. HMGB1 plays a major role incellular processes as it is involved in both the mitophagy process and functions as a pro-inflammatoryprotein. MPTP administration in adult zebrafish activated mitophagy and inflammatory signaling,highlighting the significant role of HMGB1 as a mediator in both processes and further emphasizingits significant contribution to PD pathogenesis Multidisciplinary Digital Publishing Institute (MDPI) 2023-07-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/105633/7/105633_Mitophagy%20a%20bridge%20linking%20HMGB1%20and%20Parkinson%E2%80%99s%20disease.pdf application/pdf en http://irep.iium.edu.my/105633/13/105633_Mitophagy%20a%20bridge%20linking%20HMGB1%20and%20Parkinson%E2%80%99s%20disease_Scopus.pdf Razali, Khairiah and Mohd Nasir, Mohd Hamzah and Kumar, Jaya and Mohamed, Wael Mohamed Yousef (2023) Mitophagy: a bridge linking HMGB1 and Parkinson’s disease using adult zebrafish as a model organism. Brain Sciences, 13 (7). pp. 1-15. E-ISSN 2076-3425 https://www.mdpi.com/2076-3425/13/7/1076/pdf?version=1689487671 10.3390/brainsci13071076 |
institution |
Universiti Islam Antarabangsa Malaysia |
building |
IIUM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
International Islamic University Malaysia |
content_source |
IIUM Repository (IREP) |
url_provider |
http://irep.iium.edu.my/ |
language |
English English |
topic |
R Medicine (General) |
spellingShingle |
R Medicine (General) Razali, Khairiah Mohd Nasir, Mohd Hamzah Kumar, Jaya Mohamed, Wael Mohamed Yousef Mitophagy: a bridge linking HMGB1 and Parkinson’s disease using adult zebrafish as a model organism |
description |
High-mobility group box 1 (HMGB1) has been implicated as a key player in two criticalfactors of Parkinson’s disease (PD): mitochondrial dysfunction and neuroinflammation. However, thespecific role of HMGB1 in PD remains elusive. We investigated the effect of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administration on mitochondrial dysfunction and HMGB1-associatedinflammatory genes as well as locomotor activity in zebrafish, aiming to elucidate the role of HMGB1in PD. Adult zebrafish received MPTP injections, and locomotor activity was measured at 24- and48-h post-administration. Gene expression levels related to mitophagy (fis1, pink1, and park2) andHMGB1-mediated inflammation (hmgb1, tlr4, and nfkb) were quantified through RT-qPCR analysis.Following MPTP injection, the significant increase in transcript levels of fis1, pink1, and park2 in-dicated notable changes in PINK1/Parkin mitophagy, while the upregulation of hmgb1, tlr4, andnfkb genes pointed to the activation of the HMGB1/TLR4/NFκB inflammatory pathway. Further-more, MPTP-injected zebrafish exhibited decreased locomotor activity, evident through reduceddistance travelled, mean speed, and increased freezing durations. HMGB1 plays a major role incellular processes as it is involved in both the mitophagy process and functions as a pro-inflammatoryprotein. MPTP administration in adult zebrafish activated mitophagy and inflammatory signaling,highlighting the significant role of HMGB1 as a mediator in both processes and further emphasizingits significant contribution to PD pathogenesis |
format |
Article |
author |
Razali, Khairiah Mohd Nasir, Mohd Hamzah Kumar, Jaya Mohamed, Wael Mohamed Yousef |
author_facet |
Razali, Khairiah Mohd Nasir, Mohd Hamzah Kumar, Jaya Mohamed, Wael Mohamed Yousef |
author_sort |
Razali, Khairiah |
title |
Mitophagy: a bridge linking HMGB1 and Parkinson’s disease using adult zebrafish as a model organism |
title_short |
Mitophagy: a bridge linking HMGB1 and Parkinson’s disease using adult zebrafish as a model organism |
title_full |
Mitophagy: a bridge linking HMGB1 and Parkinson’s disease using adult zebrafish as a model organism |
title_fullStr |
Mitophagy: a bridge linking HMGB1 and Parkinson’s disease using adult zebrafish as a model organism |
title_full_unstemmed |
Mitophagy: a bridge linking HMGB1 and Parkinson’s disease using adult zebrafish as a model organism |
title_sort |
mitophagy: a bridge linking hmgb1 and parkinson’s disease using adult zebrafish as a model organism |
publisher |
Multidisciplinary Digital Publishing Institute (MDPI) |
publishDate |
2023 |
url |
http://irep.iium.edu.my/105633/7/105633_Mitophagy%20a%20bridge%20linking%20HMGB1%20and%20Parkinson%E2%80%99s%20disease.pdf http://irep.iium.edu.my/105633/13/105633_Mitophagy%20a%20bridge%20linking%20HMGB1%20and%20Parkinson%E2%80%99s%20disease_Scopus.pdf http://irep.iium.edu.my/105633/ https://www.mdpi.com/2076-3425/13/7/1076/pdf?version=1689487671 |
_version_ |
1800081762112503808 |