Emulsification-assisted spectroscopic analysis of black seed oil in alginate beads: method development and validation
This study aimed to develop and validate an ultrasonication-aided UV-Vis spectroscopy method for quantifying black seed oil (BSO) in alginate beads. The method utilized sonication at 10% power rate for 120 seconds to stabilize the emulsion droplet size of 10 mL of the emulsion diluents derived fr...
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2023
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my.iium.irep.1063182023-09-05T03:39:22Z http://irep.iium.edu.my/106318/ Emulsification-assisted spectroscopic analysis of black seed oil in alginate beads: method development and validation Alkhatib, Hamzeh Mohamed, Farahidah Mohmad Sabere, Awis Sukarni Choukaife, Hazem Doolaanea, Abd Almonem Q Science (General) RS192 Materia Medica-Pharmaceutical Technology This study aimed to develop and validate an ultrasonication-aided UV-Vis spectroscopy method for quantifying black seed oil (BSO) in alginate beads. The method utilized sonication at 10% power rate for 120 seconds to stabilize the emulsion droplet size of 10 mL of the emulsion diluents derived from breaking BSO-alginate beads in phosphate buffer. Absorbance measurements were taken at 400 nm, which was found to be the optimal wavelength for measuring the absorbance of turbid emulsion diluents. The method showed excellent linearity between 0.8 - 5 mg/mL (R2 = 0.9977), with Limit of Detection (LOD) and Limit of Quantification (LOQ) values of 0.082 and 0.249 mg/mL, respectively. The method also exhibited good accuracy and precision, with recovery percentages ranging from 98.02 - 101.9% (RSD = 0.15 - 1.24%) and intra-day/inter-day precision with RSD less than 2%. These findings suggest that the ultrasonicationaided spectroscopy method is a quick, accurate, and precise way to quantify encapsulated oils and could be useful for oils analysis during R&D, In Process Quality Control (IPQC), and Quality Control (QC) tests, as well as in other applications such as industrial manufacturing and academic research. Taylor & Francis 2023-08-17 Article PeerReviewed application/pdf en http://irep.iium.edu.my/106318/1/106318_Emulsification-assisted%20spectroscopic%20analysis.pdf application/pdf en http://irep.iium.edu.my/106318/2/106318_Emulsification-assisted%20spectroscopic%20analysis_SCOPUS.pdf Alkhatib, Hamzeh and Mohamed, Farahidah and Mohmad Sabere, Awis Sukarni and Choukaife, Hazem and Doolaanea, Abd Almonem (2023) Emulsification-assisted spectroscopic analysis of black seed oil in alginate beads: method development and validation. Analytical Chemistry Letters, 13 (3). pp. 234-243. ISSN 2229-7928 E-ISSN 2230-7532 https://www.tandfonline.com/doi/abs/10.1080/22297928.2023.2243276 10.1080/22297928.2023.2243276 |
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Q Science (General) RS192 Materia Medica-Pharmaceutical Technology Alkhatib, Hamzeh Mohamed, Farahidah Mohmad Sabere, Awis Sukarni Choukaife, Hazem Doolaanea, Abd Almonem Emulsification-assisted spectroscopic analysis of black seed oil in alginate beads: method development and validation |
description |
This study aimed to develop and validate an ultrasonication-aided UV-Vis
spectroscopy method for quantifying black seed oil (BSO) in alginate beads. The
method utilized sonication at 10% power rate for 120 seconds to stabilize the
emulsion droplet size of 10 mL of the emulsion diluents derived from breaking
BSO-alginate beads in phosphate buffer. Absorbance measurements were taken
at 400 nm, which was found to be the optimal wavelength for measuring the
absorbance of turbid emulsion diluents. The method showed excellent linearity
between 0.8 - 5 mg/mL (R2
= 0.9977), with Limit of Detection (LOD) and Limit
of Quantification (LOQ) values of 0.082 and 0.249 mg/mL, respectively. The
method also exhibited good accuracy and precision, with recovery percentages
ranging from 98.02 - 101.9% (RSD = 0.15 - 1.24%) and intra-day/inter-day
precision with RSD less than 2%. These findings suggest that the ultrasonicationaided spectroscopy method is a quick, accurate, and precise way to quantify
encapsulated oils and could be useful for oils analysis during R&D, In Process
Quality Control (IPQC), and Quality Control (QC) tests, as well as in other
applications such as industrial manufacturing and academic research. |
format |
Article |
author |
Alkhatib, Hamzeh Mohamed, Farahidah Mohmad Sabere, Awis Sukarni Choukaife, Hazem Doolaanea, Abd Almonem |
author_facet |
Alkhatib, Hamzeh Mohamed, Farahidah Mohmad Sabere, Awis Sukarni Choukaife, Hazem Doolaanea, Abd Almonem |
author_sort |
Alkhatib, Hamzeh |
title |
Emulsification-assisted spectroscopic analysis of black seed oil in alginate beads: method development and validation |
title_short |
Emulsification-assisted spectroscopic analysis of black seed oil in alginate beads: method development and validation |
title_full |
Emulsification-assisted spectroscopic analysis of black seed oil in alginate beads: method development and validation |
title_fullStr |
Emulsification-assisted spectroscopic analysis of black seed oil in alginate beads: method development and validation |
title_full_unstemmed |
Emulsification-assisted spectroscopic analysis of black seed oil in alginate beads: method development and validation |
title_sort |
emulsification-assisted spectroscopic analysis of black seed oil in alginate beads: method development and validation |
publisher |
Taylor & Francis |
publishDate |
2023 |
url |
http://irep.iium.edu.my/106318/1/106318_Emulsification-assisted%20spectroscopic%20analysis.pdf http://irep.iium.edu.my/106318/2/106318_Emulsification-assisted%20spectroscopic%20analysis_SCOPUS.pdf http://irep.iium.edu.my/106318/ https://www.tandfonline.com/doi/abs/10.1080/22297928.2023.2243276 |
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