Goniothalamus umbrosus induces cell cycle arrest in oral squamous cell carcinoma cell line
Abstract Oral squamous cell carcinoma (OSCC) accounts for more than 90% of all oral cancer cases and is often connected with poor prognosis and elevated mortality rates. Current treatment modality is usually accompanied by significant side effects emphasizing the urgent need for alternative appr...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Ektodermal Displazi Grubu
2024
|
| Subjects: | |
| Online Access: | http://irep.iium.edu.my/114778/18/114778_%20Goniothalamus%20umbrosus%20induces.pdf http://irep.iium.edu.my/114778/24/114778_%20Goniothalamus%20umbrosus%20induces_Scopus.pdf http://irep.iium.edu.my/114778/ https://www.jidmr.com/journal/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Institution: | Universiti Islam Antarabangsa Malaysia |
| Language: | English English |
| Summary: | Abstract
Oral squamous cell carcinoma (OSCC) accounts for more than 90% of all oral cancer cases
and is often connected with poor prognosis and elevated mortality rates. Current treatment modality
is usually accompanied by significant side effects emphasizing the urgent need for alternative
approaches.
Goniothalamus umbrosus is traditionally consumed by cancer patients to fight against tumor
growth. Research on anti-cancer effect of G.umbrosus in oral cancer is lacking. This study aimed to
investigate cytotoxic effect of G.umbrosus in OSCC (HSC-3) and its impact on cell cycle while
comparing its activity against human gingival fibroblast cells (HGF). Cytotoxic effect was evaluated
by MTT assay. Cell cycle analysis was conducted by staining G.umbrosus treated cells with
propidium iodide and analyzed with flowcytometer. MTT assay revealed that G.umbrosus exerted
moderate and low cytotoxicity against HSC-3 and HGF cells with IC50 values of 176 μg/ml and
337.83 μg/ml, respectively. Cell cycle analysis depicted that G.umbrosus treated HSC-3 was
significantly arrested at G2/M phase (30.27%) compared to control (18.53%). These findings
revealed that G.umbrosus was selective in inhibiting viability of oral cancer cells yet not detrimental
to normal cells.
This study offered a theoretical reference for identifying potential anti-cancer agents in
developing alternative oral cancer therapies.
Experimental article (J Int Dent Med Res 2024; 17(3): 996-999) |
|---|