Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium
This paper explores the effectiveness of Flinders Technology Associates(FTA) card, with a focus on the role of FTA card application in storing oil palm DNA for PCR analysis. The study underscores the importance of optimising the washing steps of FTA card and validation o...
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my.iium.irep.1164682024-12-06T02:11:32Z http://irep.iium.edu.my/116468/ Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium Mohd Asri, Nurul Ain Shahira Zulkifli, Amirul Akmal Midin, Mohd Razik Wan Hanafi, Wan Nurhayati QH426 Genetics This paper explores the effectiveness of Flinders Technology Associates(FTA) card, with a focus on the role of FTA card application in storing oil palm DNA for PCR analysis. The study underscores the importance of optimising the washing steps of FTA card and validation of PCR amplification of using optimised washing steps towards oil palm leaves. Therefore, the study specifically examines practices for FTA washing method for oil palm sample which is crucial and necessary in obtaining promising results. The optimised washing steps such adding centrifugation while washing the FTA cards matters and has proven to enhance the band intensity of the PCR amplification results. An optimised, proper and accurate first-basis fundamental steps will contribute the value of SSR fingerprinting as a molecular detection for Elaeis guineensis, highlighting the potential of genetic markers in oil palm research. Penerbit UTHM 2024-11-21 Article PeerReviewed application/pdf en http://irep.iium.edu.my/116468/7/116468_Microsatellite%20%28mEgCIR3808%29%20analysis%20in%20oil%20palm.pdf Mohd Asri, Nurul Ain Shahira and Zulkifli, Amirul Akmal and Midin, Mohd Razik and Wan Hanafi, Wan Nurhayati (2024) Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium. International Journal of Integrated Engineering, 16 (8). pp. 80-90. ISSN 2229-838X E-ISSN 2600-7916 https://publisher.uthm.edu.my/ojs/index.php/ijie/article/view/16721/6855 |
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QH426 Genetics Mohd Asri, Nurul Ain Shahira Zulkifli, Amirul Akmal Midin, Mohd Razik Wan Hanafi, Wan Nurhayati Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium |
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This paper explores the effectiveness of Flinders Technology Associates(FTA) card, with a focus on the role of FTA card application in storing oil palm DNA for PCR analysis. The study underscores the importance of optimising the washing steps of FTA card and validation of PCR amplification of using optimised washing steps towards oil palm leaves. Therefore, the study specifically examines practices for FTA washing method for oil palm sample which is crucial and necessary in obtaining promising results. The optimised washing steps such adding centrifugation while washing the FTA cards matters and has proven to enhance the band intensity of the PCR amplification results. An optimised, proper and accurate first-basis fundamental steps will contribute the value of SSR fingerprinting as a molecular detection for Elaeis guineensis, highlighting the potential of genetic markers in oil palm research. |
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Article |
author |
Mohd Asri, Nurul Ain Shahira Zulkifli, Amirul Akmal Midin, Mohd Razik Wan Hanafi, Wan Nurhayati |
author_facet |
Mohd Asri, Nurul Ain Shahira Zulkifli, Amirul Akmal Midin, Mohd Razik Wan Hanafi, Wan Nurhayati |
author_sort |
Mohd Asri, Nurul Ain Shahira |
title |
Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium |
title_short |
Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium |
title_full |
Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium |
title_fullStr |
Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium |
title_full_unstemmed |
Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium |
title_sort |
microsatellite (megcir3808) analysis in oil palm: an optimisation of a direct pcr-based using fta card as a storage medium |
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Penerbit UTHM |
publishDate |
2024 |
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http://irep.iium.edu.my/116468/7/116468_Microsatellite%20%28mEgCIR3808%29%20analysis%20in%20oil%20palm.pdf http://irep.iium.edu.my/116468/ https://publisher.uthm.edu.my/ojs/index.php/ijie/article/view/16721/6855 |
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