Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium

This paper explores the effectiveness of Flinders Technology Associates(FTA) card, with a focus on the role of FTA card application in storing oil palm DNA for PCR analysis. The study underscores the importance of optimising the washing steps of FTA card and validation o...

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Main Authors: Mohd Asri, Nurul Ain Shahira, Zulkifli, Amirul Akmal, Midin, Mohd Razik, Wan Hanafi, Wan Nurhayati
Format: Article
Language:English
Published: Penerbit UTHM 2024
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Online Access:http://irep.iium.edu.my/116468/7/116468_Microsatellite%20%28mEgCIR3808%29%20analysis%20in%20oil%20palm.pdf
http://irep.iium.edu.my/116468/
https://publisher.uthm.edu.my/ojs/index.php/ijie/article/view/16721/6855
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Institution: Universiti Islam Antarabangsa Malaysia
Language: English
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spelling my.iium.irep.1164682024-12-06T02:11:32Z http://irep.iium.edu.my/116468/ Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium Mohd Asri, Nurul Ain Shahira Zulkifli, Amirul Akmal Midin, Mohd Razik Wan Hanafi, Wan Nurhayati QH426 Genetics This paper explores the effectiveness of Flinders Technology Associates(FTA) card, with a focus on the role of FTA card application in storing oil palm DNA for PCR analysis. The study underscores the importance of optimising the washing steps of FTA card and validation of PCR amplification of using optimised washing steps towards oil palm leaves. Therefore, the study specifically examines practices for FTA washing method for oil palm sample which is crucial and necessary in obtaining promising results. The optimised washing steps such adding centrifugation while washing the FTA cards matters and has proven to enhance the band intensity of the PCR amplification results. An optimised, proper and accurate first-basis fundamental steps will contribute the value of SSR fingerprinting as a molecular detection for Elaeis guineensis, highlighting the potential of genetic markers in oil palm research. Penerbit UTHM 2024-11-21 Article PeerReviewed application/pdf en http://irep.iium.edu.my/116468/7/116468_Microsatellite%20%28mEgCIR3808%29%20analysis%20in%20oil%20palm.pdf Mohd Asri, Nurul Ain Shahira and Zulkifli, Amirul Akmal and Midin, Mohd Razik and Wan Hanafi, Wan Nurhayati (2024) Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium. International Journal of Integrated Engineering, 16 (8). pp. 80-90. ISSN 2229-838X E-ISSN 2600-7916 https://publisher.uthm.edu.my/ojs/index.php/ijie/article/view/16721/6855
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QH426 Genetics
spellingShingle QH426 Genetics
Mohd Asri, Nurul Ain Shahira
Zulkifli, Amirul Akmal
Midin, Mohd Razik
Wan Hanafi, Wan Nurhayati
Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium
description This paper explores the effectiveness of Flinders Technology Associates(FTA) card, with a focus on the role of FTA card application in storing oil palm DNA for PCR analysis. The study underscores the importance of optimising the washing steps of FTA card and validation of PCR amplification of using optimised washing steps towards oil palm leaves. Therefore, the study specifically examines practices for FTA washing method for oil palm sample which is crucial and necessary in obtaining promising results. The optimised washing steps such adding centrifugation while washing the FTA cards matters and has proven to enhance the band intensity of the PCR amplification results. An optimised, proper and accurate first-basis fundamental steps will contribute the value of SSR fingerprinting as a molecular detection for Elaeis guineensis, highlighting the potential of genetic markers in oil palm research.
format Article
author Mohd Asri, Nurul Ain Shahira
Zulkifli, Amirul Akmal
Midin, Mohd Razik
Wan Hanafi, Wan Nurhayati
author_facet Mohd Asri, Nurul Ain Shahira
Zulkifli, Amirul Akmal
Midin, Mohd Razik
Wan Hanafi, Wan Nurhayati
author_sort Mohd Asri, Nurul Ain Shahira
title Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium
title_short Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium
title_full Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium
title_fullStr Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium
title_full_unstemmed Microsatellite (mEgCIR3808) analysis in oil palm: an optimisation of a direct PCR-based using FTA card as a storage medium
title_sort microsatellite (megcir3808) analysis in oil palm: an optimisation of a direct pcr-based using fta card as a storage medium
publisher Penerbit UTHM
publishDate 2024
url http://irep.iium.edu.my/116468/7/116468_Microsatellite%20%28mEgCIR3808%29%20analysis%20in%20oil%20palm.pdf
http://irep.iium.edu.my/116468/
https://publisher.uthm.edu.my/ojs/index.php/ijie/article/view/16721/6855
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