Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines

Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines

Fucoxanthin has been successfully isolated from species of Malaysian brown seaweed, namely Padina australis. The purity of the fucoxanthin is >98% as indicated by high performance liquid chromatography analysis. This seaweed also contains a considerable amount of unsaturated fatty acids. Thirtee...

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Main Authors: Jaswir, Irwandi, Noviendri, Dedi, Mohd. Salleh, Hamzah, Bakhtiar, M. Taher, Kazuo , Miyashita
Format: Article
Language:English
Published: Academic Journals 2011
Subjects:
TP248.13 Biotechnology
Online Access:http://irep.iium.edu.my/12109/1/Jaswir_et_al_2_isolation.pdf
http://irep.iium.edu.my/12109/
http://www.academicjournals.org/AJB
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Institution: Universiti Islam Antarabangsa Malaysia
Language: English
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spelling my.iium.irep.121092021-07-09T02:48:44Z http://irep.iium.edu.my/12109/ Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines Jaswir, Irwandi Noviendri, Dedi Mohd. Salleh, Hamzah Bakhtiar, M. Taher Kazuo , Miyashita TP248.13 Biotechnology Fucoxanthin has been successfully isolated from species of Malaysian brown seaweed, namely Padina australis. The purity of the fucoxanthin is >98% as indicated by high performance liquid chromatography analysis. This seaweed also contains a considerable amount of unsaturated fatty acids. Thirteen fatty acids were detected with gas chromatography. However, fatty acid methyl ester (FAMEs) of eicosapentanoic acid (C20:5n-3), arachidonic acid (C20:4n-6), linoleic acid (C18:2n-6) and alpha-linolenic acid (C18:3n-3) contents of P. australis were found to be 2.06, 9.50, 6.37, and 2.83%, respectively. For saturated fatty acids, palmitic acid (C16:0) was found to be the major fatty acid with about 23.97%. Furthermore, data obtained from the methyl thiazolyl tetrazolium (MTT) assay indicated that fucoxanthin reduced the viability of H1299 cell lines, showing an IC50 value of 2.45 mM. Academic Journals 2011-12-16 Article PeerReviewed application/pdf en http://irep.iium.edu.my/12109/1/Jaswir_et_al_2_isolation.pdf Jaswir, Irwandi and Noviendri, Dedi and Mohd. Salleh, Hamzah and Bakhtiar, M. Taher and Kazuo , Miyashita (2011) Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines. African Journal of Biotechnology, 10 (81). pp. 18855-18862. ISSN 1684–5315 http://www.academicjournals.org/AJB DOI: 10.5897/AJB11.2765
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP248.13 Biotechnology
spellingShingle TP248.13 Biotechnology
Jaswir, Irwandi
Noviendri, Dedi
Mohd. Salleh, Hamzah
Bakhtiar, M. Taher
Kazuo , Miyashita
Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines
description Fucoxanthin has been successfully isolated from species of Malaysian brown seaweed, namely Padina australis. The purity of the fucoxanthin is >98% as indicated by high performance liquid chromatography analysis. This seaweed also contains a considerable amount of unsaturated fatty acids. Thirteen fatty acids were detected with gas chromatography. However, fatty acid methyl ester (FAMEs) of eicosapentanoic acid (C20:5n-3), arachidonic acid (C20:4n-6), linoleic acid (C18:2n-6) and alpha-linolenic acid (C18:3n-3) contents of P. australis were found to be 2.06, 9.50, 6.37, and 2.83%, respectively. For saturated fatty acids, palmitic acid (C16:0) was found to be the major fatty acid with about 23.97%. Furthermore, data obtained from the methyl thiazolyl tetrazolium (MTT) assay indicated that fucoxanthin reduced the viability of H1299 cell lines, showing an IC50 value of 2.45 mM.
format Article
author Jaswir, Irwandi
Noviendri, Dedi
Mohd. Salleh, Hamzah
Bakhtiar, M. Taher
Kazuo , Miyashita
author_facet Jaswir, Irwandi
Noviendri, Dedi
Mohd. Salleh, Hamzah
Bakhtiar, M. Taher
Kazuo , Miyashita
author_sort Jaswir, Irwandi
title Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines
title_short Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines
title_full Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines
title_fullStr Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines
title_full_unstemmed Isolation of fucoxanthin and fatty acids analysis of Padina australis and cytotoxic effect of fucoxanthin on human lung cancer (H1299) cell lines
title_sort isolation of fucoxanthin and fatty acids analysis of padina australis and cytotoxic effect of fucoxanthin on human lung cancer (h1299) cell lines
publisher Academic Journals
publishDate 2011
url http://irep.iium.edu.my/12109/1/Jaswir_et_al_2_isolation.pdf
http://irep.iium.edu.my/12109/
http://www.academicjournals.org/AJB
_version_ 1705056436186251264

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