Gene expression of AnnAt1 in different developmental stages of Arabidopsis thaliana exposed to Pb

• Main Authors: Phang, Ing Chia, Wan Zakaria, Wan Nor Adibah, Leung, David W. M.
• Format: Conference or Workshop Item
• Language: English
• Published: 2012
• Subjects: Q Science (General)
• Online Access: http://irep.iium.edu.my/26410/1/Poster_1422.pdf; http://irep.iium.edu.my/26410/; http://www.iium.edu.my/irie/12/
• Institution: Universiti Islam Antarabangsa Malaysia

The toxicity of Pb, its widespread occurrence and persistence highlight the importance of remediation research. Phytoremediation becomes known as a potential remediation tool because it is technically and economically attractive, compared to conventional remediation technologies. However, there is a lack of Pb-related genes. Recently, annexin 1 (AnnAt1) from Arabidopsis thaliana has emerged as a key player in abiotic stresses. Annexins are reported to be related to heat shock proteins, which are the most extensively characterized stress-response genes. A recent study which uses 7-day-old seedlings discovers the participation of AnnAt1 to Pb. Since the expression of AnnAt1 may vary depending on tissue sources, developmental stages, length of exposure, and techniques used, a further experiment was conducted. A real-time PCR study was used to assess the expression of AnnAt1 in different developmental stage of A. thaliana grown in Pb-containing medium.