Chemical constituents and bioactivities of garcinia prainiana

• Main Authors: On, Shamsul, Ahmad, Farediah, M Sirat , Hasnah, Bakhtiar, M. Taher
• Format: Conference or Workshop Item
• Language: English; English
• Published: 2013
• Subjects: RS Pharmacy and materia medica
• Online Access: http://irep.iium.edu.my/29479/1/ICNP_2013_shamsul.pdf; http://irep.iium.edu.my/29479/7/bio.pdf; http://irep.iium.edu.my/29479/; http://icnp2013.uitm.edu.my/v1/component/content/article/3-icnp2013.html
• Institution: Universiti Islam Antarabangsa Malaysia

Garcinia is the largest genus in Guttiferae family found in tropics as trees and shrubs. This genus is a rich source of phenolic compounds comprised of xanthones, biflavanoids and benzophenones. It also has been shown to have bioactivities such as antioxidative, antimicrobial, anti-inflammatory, cytotoxic and anti-HIV. In this study, the phytochemical investigation of the leaves and stem barks of G. prainiana and bioactivities of crude extracts and isolated compounds were carried out. The leaves and stem barks of G. prainiana collected from Kuantan, Pahang were extracted by soxhlet extractor with hexane, dichloromethane and methanol. The crude extracts were purified by column chromatography to yield pure compounds which were characterized spectroscopically using IR, NMR (1D and 2D), UV and MS. Fractionation and purification of the hexane extract of leaves yielded squalene, friedelin, 3β-hydroxyeupha-5,22-diene and 3β-acetoxyeupha-5,22-diene. The methanol extract of the stem barks afforded biflavanoids characterized as morelloflavone, O-methylfukugetin, amentoflavone, 4’’’-methoxyamentoflavone and volkensiflavone. The total phenolic content studies were carried out on the crude extracts by using Folin-Ciocalteau reagent. The methanol extract of leaves and stem barks showed the highest total phenolic content expressed as gallic acid and (±)-catechin equivalents. These extracts also showed the highest value of ascorbic acid and butylated hydroxytoluene (BHT) equivalents on forming the phosphomolybdenum complex in the total antioxidant assay. The antioxidant assay on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical showed that the methanol extract of stem barks had the highest free radical scavenging activity with IC50 value 74.5 µg/mL while the isolated compound, morelloflavone revealed a strong free radical scavenging activity with IC50 value 15.7 µg/mL. The antibacterial assay was done by disc diffusion method, followed by minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) determinations. The crude methanol extract of stem bark displayed moderate antibacterial activity against Gram-negative bacteria Pseudomonas putida, Escherichia coli, Pseudomonas aeruginosa and Gram-positive bacteria Basillus subtilis and the isolated compound, morelloflavone also showed moderate antibacterial activity against Pseudomonas putida and Pseudomonas aeruginosa with MIC value 900 µg/L. The cytotoxic activities of the isolated compounds were evaluated using the MTT assay against H1299 and A549 human lung cancer cells. The results showed that squalene had strong cytotoxity against H1299 and A549 cancer cells with IC50 value 23.0 µg/mL and 74.0 µg/mL respectively, while the other compounds were inactive against the tested cell lines.