Characterization of endothelial cells using electrochemical impedance spectroscopy

• Main Authors: Liu, Fei, Arifuzzaman, S. M., Nordin, Anis Nurashikin, Spray, David, Voiculescu, Ioana
• Format: Conference or Workshop Item
• Language: English
• Published: 2010
• Subjects: TK7885 Computer engineering
• Online Access: http://irep.iium.edu.my/3702/1/3.pdf; http://irep.iium.edu.my/3702/; http://ieeexplore.ieee.org/xpls/abs_all.jsp?arnumber=5774824
• Institution: Universiti Islam Antarabangsa Malaysia

In this paper we report on the electrical impedance spectroscopy characterization of endothelial cell lines (RFPEC). For electrical cell-substrate impedance sensing (ECIS) of the endothelial cells a commercially available eight-well cell culture impedance array (ECIS-8W1E) was used. The impedance measurements were recorded with cell culture medium (without cells) and with endothelial cell layer in the culture medium over a frequency range from 100 Hz to 100 kHz. The impedance measurements were compared to the equivalent circuit model. The impedance measurements of endothelia cells were also simulated using COMSOL Multiphysics #x2122;, a commercially available modeling package.