Expression of segment a of infectious bursal disease virus in pichia pastoris

Recombinant plasmid containing segment A open reading frame 2 (ORF2) gene of infectious bursal disease virus (IBDV) of a very virulent subtype from local outbreak (strain 3529/92) was constructed. The gene encoding the IBDV structural polyprotein (N-VP2-VP3-VP4-C) was inserted into an expression vec...

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Main Authors: Ghazali, Mohd. Shahrin, Ahmad Raus, Raha, Amin, Noriha M., Shohaimi, Syamsiah A., Abu Bakar, Farah D., Zainuddin, Norafiza, Jimat, Dzun Noraini, Nor Muhammad, Nor Azlin Alia
Format: Article
Language:English
English
Published: Journal of Pure and Applied Microbiology 2014
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Online Access:http://irep.iium.edu.my/39365/2/Expression_of_Segment_A_gene.pdf
http://irep.iium.edu.my/39365/4/39365_Expression%20of%20segment_Scopus.pdf
http://irep.iium.edu.my/39365/
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Institution: Universiti Islam Antarabangsa Malaysia
Language: English
English
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spelling my.iium.irep.393652024-06-21T01:44:02Z http://irep.iium.edu.my/39365/ Expression of segment a of infectious bursal disease virus in pichia pastoris Ghazali, Mohd. Shahrin Ahmad Raus, Raha Amin, Noriha M. Shohaimi, Syamsiah A. Abu Bakar, Farah D. Zainuddin, Norafiza Jimat, Dzun Noraini Nor Muhammad, Nor Azlin Alia Q Science (General) Recombinant plasmid containing segment A open reading frame 2 (ORF2) gene of infectious bursal disease virus (IBDV) of a very virulent subtype from local outbreak (strain 3529/92) was constructed. The gene encoding the IBDV structural polyprotein (N-VP2-VP3-VP4-C) was inserted into an expression vector, pPICZ prior to its transformation into Pichia pastoris by electroporation. After the induction of P. pastoris transformant with 0.5% methanol, the production of IBDV polyprotein was observed using Western blot. In P. pastoris, co- or post-translational processing of the large polyprotein occurred, generating a stable C-terminal product (VP3) of correct size, but without any detectable N-terminal product (VP2). The failure to observe the VP2 protein in Western blot analysis was probably due to the conformational epitope problem. Journal of Pure and Applied Microbiology 2014-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/39365/2/Expression_of_Segment_A_gene.pdf application/pdf en http://irep.iium.edu.my/39365/4/39365_Expression%20of%20segment_Scopus.pdf Ghazali, Mohd. Shahrin and Ahmad Raus, Raha and Amin, Noriha M. and Shohaimi, Syamsiah A. and Abu Bakar, Farah D. and Zainuddin, Norafiza and Jimat, Dzun Noraini and Nor Muhammad, Nor Azlin Alia (2014) Expression of segment a of infectious bursal disease virus in pichia pastoris. Journal of Pure and Applied Microbiology, 8 spl. pp. 875-879. ISSN 0973-7510
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic Q Science (General)
spellingShingle Q Science (General)
Ghazali, Mohd. Shahrin
Ahmad Raus, Raha
Amin, Noriha M.
Shohaimi, Syamsiah A.
Abu Bakar, Farah D.
Zainuddin, Norafiza
Jimat, Dzun Noraini
Nor Muhammad, Nor Azlin Alia
Expression of segment a of infectious bursal disease virus in pichia pastoris
description Recombinant plasmid containing segment A open reading frame 2 (ORF2) gene of infectious bursal disease virus (IBDV) of a very virulent subtype from local outbreak (strain 3529/92) was constructed. The gene encoding the IBDV structural polyprotein (N-VP2-VP3-VP4-C) was inserted into an expression vector, pPICZ prior to its transformation into Pichia pastoris by electroporation. After the induction of P. pastoris transformant with 0.5% methanol, the production of IBDV polyprotein was observed using Western blot. In P. pastoris, co- or post-translational processing of the large polyprotein occurred, generating a stable C-terminal product (VP3) of correct size, but without any detectable N-terminal product (VP2). The failure to observe the VP2 protein in Western blot analysis was probably due to the conformational epitope problem.
format Article
author Ghazali, Mohd. Shahrin
Ahmad Raus, Raha
Amin, Noriha M.
Shohaimi, Syamsiah A.
Abu Bakar, Farah D.
Zainuddin, Norafiza
Jimat, Dzun Noraini
Nor Muhammad, Nor Azlin Alia
author_facet Ghazali, Mohd. Shahrin
Ahmad Raus, Raha
Amin, Noriha M.
Shohaimi, Syamsiah A.
Abu Bakar, Farah D.
Zainuddin, Norafiza
Jimat, Dzun Noraini
Nor Muhammad, Nor Azlin Alia
author_sort Ghazali, Mohd. Shahrin
title Expression of segment a of infectious bursal disease virus in pichia pastoris
title_short Expression of segment a of infectious bursal disease virus in pichia pastoris
title_full Expression of segment a of infectious bursal disease virus in pichia pastoris
title_fullStr Expression of segment a of infectious bursal disease virus in pichia pastoris
title_full_unstemmed Expression of segment a of infectious bursal disease virus in pichia pastoris
title_sort expression of segment a of infectious bursal disease virus in pichia pastoris
publisher Journal of Pure and Applied Microbiology
publishDate 2014
url http://irep.iium.edu.my/39365/2/Expression_of_Segment_A_gene.pdf
http://irep.iium.edu.my/39365/4/39365_Expression%20of%20segment_Scopus.pdf
http://irep.iium.edu.my/39365/
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