The use of fibrin and poly(lactic-co-glycolic acid) hybrid scaffold for articular cartilage tissue engineering: an in vivo analysis
Our preliminary results indicated that fibrin and poly(lactic co-glycolic acid) (PLGA) hybrid scaffold promoted early chondrogenesis of articular cartilage constructs in vitro. The aim of this study was to evaluate in vivo cartilaginous tissue formation by chondrocyte-seeded fibrin/PLGA hybrid scaff...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
AO Research Institute Davos
2008
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/41404/1/v015a04.pdf http://irep.iium.edu.my/41404/ http://www.ecmjournal.org/journal/papers/vol015/vol015a04.php |
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| Institution: | Universiti Islam Antarabangsa Malaysia |
| Language: | English |
| Summary: | Our preliminary results indicated that fibrin and poly(lactic co-glycolic acid) (PLGA) hybrid scaffold promoted early chondrogenesis of articular cartilage constructs in vitro. The aim of this study was to evaluate in vivo cartilaginous tissue formation by chondrocyte-seeded fibrin/PLGA hybrid scaffolds. PLGA scaffolds were soaked carefully, in chondrocyte-fibrin suspension, and polymerized by dropping thrombin-calcium chloride (CaCl2) solution.PLGA-seeded chondrocytes were used as a control.
Resulting constructs were implanted subcutaneously, at the
dorsum of nude mice, for 4 weeks. Macroscopic observation, histological evaluation, gene expression and
sulphated-glycosaminoglycan (sGAG) analyses were
performed at each time point of 1, 2 and 4 weeks postimplantation.Cartilaginous tissue formation in fibrin/PLGA hybrid construct was confirmed by the presence of lacunae and cartilage-isolated cells embedded within basophilic ground substance. Presence of proteoglycan and
glycosaminoglycan (GAG) in fibrin/PLGA hybrid
constructs was confirmed by positive Safranin O and Alcian
Blue staining. Collagen type II exhibited intense
immunopositivity at the pericellular matrices.
Chondrogenic properties were further demonstrated by the
expression of gene encoded cartilage-specific markers,
collagen type II and aggrecan core protein. The sGAG
production in fibrin/PLGA hybrid constructs was higher
than in the PLGA group. In conclusion, fibrin/PLGA hybrid
scaffold promotes cartilaginous tissue formation in vivo and
may serve as a potential cell delivery vehicle and a structural basis for articular cartilage tissue-engineering. |
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