Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds
Tissue engineering and regenerative medicine has the potential of improving the quality of life for many people. One particular area that presents an exciting challenge is the regeneration of intervertebral discs (IVD). To achieve this, it is necessary to identify suitable cells, biomaterial scaffol...
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Tissue Engineering and Regenerative Medicine Society of Malaysia
2014
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my.iium.irep.414202015-02-12T09:07:29Z http://irep.iium.edu.my/41420/ Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds Md Nazir, Noorhidayah T. M., Tengku Nuramirah Razak, Syahidah Mohamad, Mohd Yusof Abdul Rahman, Rozlin Mohamad Syukri, Norhamiza Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Sha'ban, Munirah R Medicine (General) Tissue engineering and regenerative medicine has the potential of improving the quality of life for many people. One particular area that presents an exciting challenge is the regeneration of intervertebral discs (IVD). To achieve this, it is necessary to identify suitable cells, biomaterial scaffolds and biologically active signalling factors for in vitro and in vivo IVD generation. This present study aimed to evaluate the cartilaginous properties of nucleus pulposus (NP) cells seeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin scaffolds by measuring sulphated glycosaminoglycan (sGAG) content and collagen type II (COL-II) gene expression. The analyses were performed at week 1, 2, and 3 in vitro. The sGAG content was higher in the PLGA/Fibrin than in the PLGA only group. The expression of COL-II was only detected at week-2 culture in PLGA/Fibrin group. This study indicates that PLGA-based scaffolds may have the potential for intervertebral discs tissue engineering. Tissue Engineering and Regenerative Medicine Society of Malaysia 2014 Article REM application/pdf en http://irep.iium.edu.my/41420/3/RR-181114-032.pdf Md Nazir, Noorhidayah and T. M., Tengku Nuramirah and Razak, Syahidah and Mohamad, Mohd Yusof and Abdul Rahman, Rozlin and Mohamad Syukri, Norhamiza and Ahmad Radzi, Muhammad Aa'zamuddin and Zulkifly, Ahmad Hafiz and Sha'ban, Munirah (2014) Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds. Regenerative Research, 3 (2). pp. 46-47. ISSN 2232-0822 (Online) http://regres.tesma.org.my/pdf/RR-181114-032.pdf |
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R Medicine (General) Md Nazir, Noorhidayah T. M., Tengku Nuramirah Razak, Syahidah Mohamad, Mohd Yusof Abdul Rahman, Rozlin Mohamad Syukri, Norhamiza Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Sha'ban, Munirah Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds |
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Tissue engineering and regenerative medicine has the potential of improving the quality of life for many people. One particular area that presents an exciting challenge is the regeneration of intervertebral discs (IVD). To achieve this, it is necessary to identify suitable cells, biomaterial scaffolds and biologically active signalling factors for in vitro and in vivo IVD generation. This present study aimed to evaluate the cartilaginous properties of nucleus pulposus (NP) cells seeded on poly(lactic-co-glycolic acid) (PLGA) with or without fibrin scaffolds by measuring sulphated glycosaminoglycan (sGAG) content and collagen type II (COL-II) gene expression. The analyses were performed at week 1, 2, and 3 in vitro. The sGAG content was higher in the PLGA/Fibrin than in the PLGA only group. The expression of COL-II was only detected at week-2 culture in PLGA/Fibrin group. This study indicates that PLGA-based scaffolds may have the potential for intervertebral discs tissue engineering. |
format |
Article |
author |
Md Nazir, Noorhidayah T. M., Tengku Nuramirah Razak, Syahidah Mohamad, Mohd Yusof Abdul Rahman, Rozlin Mohamad Syukri, Norhamiza Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Sha'ban, Munirah |
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Md Nazir, Noorhidayah T. M., Tengku Nuramirah Razak, Syahidah Mohamad, Mohd Yusof Abdul Rahman, Rozlin Mohamad Syukri, Norhamiza Ahmad Radzi, Muhammad Aa'zamuddin Zulkifly, Ahmad Hafiz Sha'ban, Munirah |
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Md Nazir, Noorhidayah |
title |
Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds |
title_short |
Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds |
title_full |
Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds |
title_fullStr |
Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds |
title_full_unstemmed |
Cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds |
title_sort |
cartilaginous properties of nucleus pulposus cells seeded on poly(lactic-co-glycolic acid)-based scaffolds |
publisher |
Tissue Engineering and Regenerative Medicine Society of Malaysia |
publishDate |
2014 |
url |
http://irep.iium.edu.my/41420/3/RR-181114-032.pdf http://irep.iium.edu.my/41420/ http://regres.tesma.org.my/pdf/RR-181114-032.pdf |
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