A hydrogel stamper with expandable height and built-in reservoirs for patterning biomolecules on 3D topologies

In this paper, we report on an expandable height hydrogel stamper with built-in reservoir that can be used to repeatedly (without frequent re-inking) stamp functional biomolecules on planar and non-planar surfaces. We have successfully patterned fluorescein isothiocyante (FITC) labeled antibodie...

Full description

Saved in:
Bibliographic Details
Main Authors: Wan Salim, Wan Wardatul Amani, Ding, Zhenweng, Ziaie, Babak
Format: Conference or Workshop Item
Language:English
Published: IEEE 2008
Subjects:
Online Access:http://irep.iium.edu.my/44332/7/44332%20A%20Hydrogel%20Stamper%20with%20Expandable%20Height.pdf
http://irep.iium.edu.my/44332/
https://ieeexplore.ieee.org/document/4433117
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Universiti Islam Antarabangsa Malaysia
Language: English
Description
Summary:In this paper, we report on an expandable height hydrogel stamper with built-in reservoir that can be used to repeatedly (without frequent re-inking) stamp functional biomolecules on planar and non-planar surfaces. We have successfully patterned fluorescein isothiocyante (FITC) labeled antibodies (antibodies for O-antigens of Bradyrhizobium japonicum made in rabbits) on planar (silicon) and nonplanar (SU8 on silicon with a height of ~ 70µm) topologies. The printed antibodies show the effectiveness of hydrogel stampers in delivering biomolecules on 3D topologies where the hydrogel could be adjusted to stamp on the sidewalls and bottom of deep trenches.