DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei

Burkholderia pseudomallei is a saprophytic Gram-negative bacillus that is found in soil and surface water. It causes the disease melioidosis, which infect humans and animals. Melioidosis can be fatal in human, where it causes fever and commonly present with pneumonia, with or without septicaemia. Me...

Full description

Saved in:
Bibliographic Details
Main Authors: Osman, Abdul Latif, Syed Abdullah, Syed Mohamed, Drahaman, Siti Marhamah, Raih, Mohd Firdaus, Muhamad Bunnori, Noraslinda, Mohamed Rehan, Aisyah
Format: Article
Language:English
Published: Universiti Teknologi Malaysia 2015
Subjects:
Online Access:http://irep.iium.edu.my/46351/1/2015_JurnalTeknologi_Abdul_Latif_et_al_AISYAH_B._Pseudomallei.pdf
http://irep.iium.edu.my/46351/
http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/6756
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Universiti Islam Antarabangsa Malaysia
Language: English
id my.iium.irep.46351
record_format dspace
spelling my.iium.irep.46351 http://irep.iium.edu.my/46351/ DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei Osman, Abdul Latif Syed Abdullah, Syed Mohamed Drahaman, Siti Marhamah Raih, Mohd Firdaus Muhamad Bunnori, Noraslinda Mohamed Rehan, Aisyah QR Microbiology Burkholderia pseudomallei is a saprophytic Gram-negative bacillus that is found in soil and surface water. It causes the disease melioidosis, which infect humans and animals. Melioidosis can be fatal in human, where it causes fever and commonly present with pneumonia, with or without septicaemia. Melioidosis is primarily endemic in Southeast Asia and Northern Australia, but has also been found in the Middle East, China and South America. It is challenging to treat melioidosis, as it is intrinsically resistant to many antibiotics, and can cause latent infection. By characterizing identified protein targets from B. pseudomallei, we can gain fundamental knowledge on how this bacterium behaves, and thus provide us strategies to combat them. We report here the recent progress of DNA amplification and cloning of four target genes from B. pseudomallei strain D286 performed in Kulliyyah of Science, IIUM. Genomic DNA of B. pseudomallei strain D286 is obtained from School of Biosciences & Biotechnology, UKM. The four target genes; BPSL1612, BPSL1618, BPSL1691 and BPSL2054 were chosen from 52 hypothetical proteins predicted to be essential in B. pseudomallei by transposon-directed insertion site sequencing (TraDIS) technique (Moule et al., 2014). All four target genes were subjected to nested PCR amplification for subsequent GatewayTM cloning protocols, expression and purification studies. Universiti Teknologi Malaysia 2015-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/46351/1/2015_JurnalTeknologi_Abdul_Latif_et_al_AISYAH_B._Pseudomallei.pdf Osman, Abdul Latif and Syed Abdullah, Syed Mohamed and Drahaman, Siti Marhamah and Raih, Mohd Firdaus and Muhamad Bunnori, Noraslinda and Mohamed Rehan, Aisyah (2015) DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei. Jurnal Teknologi, 77 (25). pp. 153-158. ISSN 2180–3722 (O), 0127–9696 (P) http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/6756
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QR Microbiology
spellingShingle QR Microbiology
Osman, Abdul Latif
Syed Abdullah, Syed Mohamed
Drahaman, Siti Marhamah
Raih, Mohd Firdaus
Muhamad Bunnori, Noraslinda
Mohamed Rehan, Aisyah
DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei
description Burkholderia pseudomallei is a saprophytic Gram-negative bacillus that is found in soil and surface water. It causes the disease melioidosis, which infect humans and animals. Melioidosis can be fatal in human, where it causes fever and commonly present with pneumonia, with or without septicaemia. Melioidosis is primarily endemic in Southeast Asia and Northern Australia, but has also been found in the Middle East, China and South America. It is challenging to treat melioidosis, as it is intrinsically resistant to many antibiotics, and can cause latent infection. By characterizing identified protein targets from B. pseudomallei, we can gain fundamental knowledge on how this bacterium behaves, and thus provide us strategies to combat them. We report here the recent progress of DNA amplification and cloning of four target genes from B. pseudomallei strain D286 performed in Kulliyyah of Science, IIUM. Genomic DNA of B. pseudomallei strain D286 is obtained from School of Biosciences & Biotechnology, UKM. The four target genes; BPSL1612, BPSL1618, BPSL1691 and BPSL2054 were chosen from 52 hypothetical proteins predicted to be essential in B. pseudomallei by transposon-directed insertion site sequencing (TraDIS) technique (Moule et al., 2014). All four target genes were subjected to nested PCR amplification for subsequent GatewayTM cloning protocols, expression and purification studies.
format Article
author Osman, Abdul Latif
Syed Abdullah, Syed Mohamed
Drahaman, Siti Marhamah
Raih, Mohd Firdaus
Muhamad Bunnori, Noraslinda
Mohamed Rehan, Aisyah
author_facet Osman, Abdul Latif
Syed Abdullah, Syed Mohamed
Drahaman, Siti Marhamah
Raih, Mohd Firdaus
Muhamad Bunnori, Noraslinda
Mohamed Rehan, Aisyah
author_sort Osman, Abdul Latif
title DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei
title_short DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei
title_full DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei
title_fullStr DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei
title_full_unstemmed DNA amplification, optimization and cloning of several target genes from Burkholderia pseudomallei
title_sort dna amplification, optimization and cloning of several target genes from burkholderia pseudomallei
publisher Universiti Teknologi Malaysia
publishDate 2015
url http://irep.iium.edu.my/46351/1/2015_JurnalTeknologi_Abdul_Latif_et_al_AISYAH_B._Pseudomallei.pdf
http://irep.iium.edu.my/46351/
http://www.jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/6756
_version_ 1643616869302665216