Antileukemogenesis of nypa fruticans on acute lymphoblastic leukemia – a preliminary study in vitro

• Main Authors: Mamat, Suhana, Fadzir, Ummu Afifah, Zainudin, Muhammad Fauzan, Rosdi, Athirah, Abdul Wahab, Ridhwan, Hashim, Ridzwan
• Format: Conference or Workshop Item
• Language: English
• Published: 2015
• Subjects: RB Pathology
• Online Access: http://irep.iium.edu.my/47178/10/47178%20ANTILEUKEMOGENESIS%20OF%20NYPA%20FRUTICANS.pdf; http://irep.iium.edu.my/47178/
• Institution: Universiti Islam Antarabangsa Malaysia

Background: Leukemia is one of the common cancers worldwide. Although the treatment options available for most type of cancers, but some of them remain incurable and the re-occurrence of disease is also common in many patients. Furthermore, modern therapy also leads to many side effects. All of these lead us to study on the possible anticancer activity of local fruit known as Nypa fruticans or locally known as nipah. Objective: This study was aimed to determine the antileukemogenesis effect of Nypa fruticans on leukemic cell lines (CCRF-CEM). Methodology: In this study, matured Nypa fruticans fruits was extracted using soxhlet apparatus. Methanol was used as the solvent for extraction. Different concentration of Nypa fruticans (3.125, 6.25, 12.5,25 & 50 µg/mL) extracts were tested on CCRF-CEM cells (lymphoblastic leukemia cell lines). Trypan blue assay was use to screen for concentration of nipah extracts that affect the viability of the cells after 24 hours of treatment. This was followed by determination of cells proliferation rate by BrdU assay. Results: The trypan blue assay showed prominent reduction in the cell viability when higher concentration of Nypa fruticans used to treat CCRF-CEM cell line. There were significant differences in concentration 12.5, 25 and 50 µg/mL compared to the control group. Furthermore, Brdu assay confirmed the decreasing trend in cell proliferation index following higher concentration of Nypa fruticans treatment on CCRF-CEM cells. Conclusion: This preliminary data suggest that, Nypa fruticans has the anti-leukemogenesis effect on acute lymphoblastic cell line.