Simulation of absorption spectrum of photosynthetic pigments of chlorella vulgaris B algae

• Main Authors: Mahmoud Ahmed, Gharib Subhi, Hassan, Taher, Othman, Rashidi, Messikh Azeddine, Azeddine
• Format: Article
• Language: English; English
• Published: Institute for Mathematical Research (INSPEM), Universiti Putra Malaysia 2016
• Subjects: T Technology (General)
• Online Access: http://irep.iium.edu.my/50717/6/50717-article.pdf; http://irep.iium.edu.my/50717/7/50717-Simulation%20of%20absorption%20spectrum%20of%20photosynthetic%20pigments%20of%20chlorella%20vulgaris%20B%20algae_SCOPUS.pdf; http://irep.iium.edu.my/50717/; http://einspem.upm.edu.my/journal/fullpaper/vol10sfeb/No11.pdf
• Institution: Universiti Islam Antarabangsa Malaysia

Absorption spectrum of Chlorella vulgaris B algae was reconstructed by simulated the absorption spectrum of the mixture under examination by a linear combination of the absorption spectra of all its constituents. A C30 reversed high performance liquid chromatography equipped with photodiode array detector (HPLC-PAD) and absorption spectrophotometer were used to separate and identify photosynthetic pigments of Chlorella vulgaris B. Spectrum-reconstruction method (SRCM) was used to calculate concentrations of total carotenoids, chlorophylls a and b. Absorption spectrum of the total pigments extracted of Chlorella vulgaris B. was reconstructed from the sum of the spectrum of individual extracted pigments taken from (HPLC-PAD), and compared using normalization with absorption spectrum of the total pigments recorded by spectrophotometer. A total of seven Pigments were separated and identified