Method for preparing plasmid DNA
A METHOD FOR PREPARING PLASMID DNA, COMPRISING THE STEPS OF CULTURING HOST CELLS CONTAINING PLASMID DNA; LYSING THE HOST CELLS TO OBTAIN A LYSATE; ISOLATING SAID PLASMID DNA FROM SAID LYSATE TO FORM ISOLATED PLASMID DNA; AND PURIFYING THE ISOLATED PLASMID DNA; CHARACTERIZED IN THAT THE LYSATE IS SUB...
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Main Authors: | , , |
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Format: | Patent |
Language: | English |
Published: |
2015
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Subjects: | |
Online Access: | http://irep.iium.edu.my/53741/1/METHOD%20FOR%20PREPARING%20PLASMID%20DNA.pdf http://irep.iium.edu.my/53741/ http://onlineip.myipo.gov.my/index.cfm/search/pt/details/id/X515563 |
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Institution: | Universiti Islam Antarabangsa Malaysia |
Language: | English |
Summary: | A METHOD FOR PREPARING PLASMID DNA, COMPRISING THE STEPS OF CULTURING HOST CELLS CONTAINING PLASMID DNA; LYSING THE HOST CELLS TO OBTAIN A LYSATE; ISOLATING SAID PLASMID DNA FROM SAID LYSATE TO FORM ISOLATED PLASMID DNA; AND PURIFYING THE ISOLATED PLASMID DNA; CHARACTERIZED IN THAT THE LYSATE IS SUBSTANTIALLY LYOPHILIZED AND SUBSEQUENTLY RESUSPENDED WITH WATER, PRIOR TO ISOLATION. (FIG 5A) |
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