Method for preparing plasmid DNA

A METHOD FOR PREPARING PLASMID DNA, COMPRISING THE STEPS OF CULTURING HOST CELLS CONTAINING PLASMID DNA; LYSING THE HOST CELLS TO OBTAIN A LYSATE; ISOLATING SAID PLASMID DNA FROM SAID LYSATE TO FORM ISOLATED PLASMID DNA; AND PURIFYING THE ISOLATED PLASMID DNA; CHARACTERIZED IN THAT THE LYSATE IS SUB...

Full description

Saved in:
Bibliographic Details
Main Authors: Mohamed, Farahidah, Doolaanea, Abd Almonem, Mansor, Nur 'Izzati
Format: Patent
Language:English
Published: 2015
Subjects:
Online Access:http://irep.iium.edu.my/53741/1/METHOD%20FOR%20PREPARING%20PLASMID%20DNA.pdf
http://irep.iium.edu.my/53741/
http://onlineip.myipo.gov.my/index.cfm/search/pt/details/id/X515563
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Universiti Islam Antarabangsa Malaysia
Language: English
Description
Summary:A METHOD FOR PREPARING PLASMID DNA, COMPRISING THE STEPS OF CULTURING HOST CELLS CONTAINING PLASMID DNA; LYSING THE HOST CELLS TO OBTAIN A LYSATE; ISOLATING SAID PLASMID DNA FROM SAID LYSATE TO FORM ISOLATED PLASMID DNA; AND PURIFYING THE ISOLATED PLASMID DNA; CHARACTERIZED IN THAT THE LYSATE IS SUBSTANTIALLY LYOPHILIZED AND SUBSEQUENTLY RESUSPENDED WITH WATER, PRIOR TO ISOLATION. (FIG 5A)