Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan

Cryptosporidium spp. are ubiquitous enteric protozoan parasites that cause diarrhoeal disease known as cryptosporidiosis. This research was conducted to find the prevalence of Cryptosporidium from dairy cattle in Kuantan, Pahang, Malaysia and to identify the genotype of Cryptosporidium by using 18S...

Full description

Saved in:
Bibliographic Details
Main Authors: Mat Yusof, Afzan, Md Isa, Muhammad Lokman
Format: Article
Language:English
English
Published: Malaysian Society of Applied Biology 2017
Subjects:
Online Access:http://irep.iium.edu.my/57533/1/Afzan%20and%20Isa%2C%20%282017%29.pdf
http://irep.iium.edu.my/57533/7/57533_Prevalence%2C%20age-related%20and%20molecular_SCOPUS.pdf
http://irep.iium.edu.my/57533/
http://mabjournal.com/images/46_2_June_2017/46_02_03.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Universiti Islam Antarabangsa Malaysia
Language: English
English
id my.iium.irep.57533
record_format dspace
spelling my.iium.irep.575332017-09-29T03:52:00Z http://irep.iium.edu.my/57533/ Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan Mat Yusof, Afzan Md Isa, Muhammad Lokman Q Science (General) Cryptosporidium spp. are ubiquitous enteric protozoan parasites that cause diarrhoeal disease known as cryptosporidiosis. This research was conducted to find the prevalence of Cryptosporidium from dairy cattle in Kuantan, Pahang, Malaysia and to identify the genotype of Cryptosporidium by using 18S rRNA gene. Besides, this study aims to investigate the association between Cryptosporidium infection and the age of dairy cattle. 375 stool specimens of dairy cattle were collected and concentrated with formal-ether concentration technique. The Cryptosporidium oocysts were detected with modified Ziehl Neelsen staining. Cryptosporidium species was identified by nested PCR amplification of 18S rRNA gene. Based on microscopic examination, 16.3% (61/375) dairy cattle were positive for Cryptosporidium infection. This research has shown that the highest prevalence of Cryptosporidium was recorded in calves with the percentage of 17.4% (12/69), followed by adult cattle and yearling with the percentage of 16.1% (29/180) and 15.9% (20/126), respectively. The findings demonstrated that there was no significant difference (p > 0.05) in Cryptosporidium infection rates by age. Molecular characterization revealed that the species of Cryptosporidium found in dairy cattle was C. ryanae. The present study suggested that proper hygiene practices must be practiced by farmers in order to control the Cryptosporidium infection. Malaysian Society of Applied Biology 2017-06 Article REM application/pdf en http://irep.iium.edu.my/57533/1/Afzan%20and%20Isa%2C%20%282017%29.pdf application/pdf en http://irep.iium.edu.my/57533/7/57533_Prevalence%2C%20age-related%20and%20molecular_SCOPUS.pdf Mat Yusof, Afzan and Md Isa, Muhammad Lokman (2017) Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan. Malaysian Applied Biology, 46 (2). pp. 21-27. ISSN 0126-8643 http://mabjournal.com/images/46_2_June_2017/46_02_03.pdf
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic Q Science (General)
spellingShingle Q Science (General)
Mat Yusof, Afzan
Md Isa, Muhammad Lokman
Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan
description Cryptosporidium spp. are ubiquitous enteric protozoan parasites that cause diarrhoeal disease known as cryptosporidiosis. This research was conducted to find the prevalence of Cryptosporidium from dairy cattle in Kuantan, Pahang, Malaysia and to identify the genotype of Cryptosporidium by using 18S rRNA gene. Besides, this study aims to investigate the association between Cryptosporidium infection and the age of dairy cattle. 375 stool specimens of dairy cattle were collected and concentrated with formal-ether concentration technique. The Cryptosporidium oocysts were detected with modified Ziehl Neelsen staining. Cryptosporidium species was identified by nested PCR amplification of 18S rRNA gene. Based on microscopic examination, 16.3% (61/375) dairy cattle were positive for Cryptosporidium infection. This research has shown that the highest prevalence of Cryptosporidium was recorded in calves with the percentage of 17.4% (12/69), followed by adult cattle and yearling with the percentage of 16.1% (29/180) and 15.9% (20/126), respectively. The findings demonstrated that there was no significant difference (p > 0.05) in Cryptosporidium infection rates by age. Molecular characterization revealed that the species of Cryptosporidium found in dairy cattle was C. ryanae. The present study suggested that proper hygiene practices must be practiced by farmers in order to control the Cryptosporidium infection.
format Article
author Mat Yusof, Afzan
Md Isa, Muhammad Lokman
author_facet Mat Yusof, Afzan
Md Isa, Muhammad Lokman
author_sort Mat Yusof, Afzan
title Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan
title_short Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan
title_full Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan
title_fullStr Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan
title_full_unstemmed Prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in Kuantan
title_sort prevalence, age-related and molecular of cryptosporidium from dairy cattle in five farms in kuantan
publisher Malaysian Society of Applied Biology
publishDate 2017
url http://irep.iium.edu.my/57533/1/Afzan%20and%20Isa%2C%20%282017%29.pdf
http://irep.iium.edu.my/57533/7/57533_Prevalence%2C%20age-related%20and%20molecular_SCOPUS.pdf
http://irep.iium.edu.my/57533/
http://mabjournal.com/images/46_2_June_2017/46_02_03.pdf
_version_ 1643615166023073792