Early response in antibacterial activity of orthopaedic metal implant coated with silver composite as future implant related infection treatment – Preliminary result

• Main Authors: Mohd Jan, Nurul Hafiza, Zulkifly, Ahmad Hafiz, Ibrahim, Mohd Zulfadzli, Shaban, Munirah
• Format: Conference or Workshop Item
• Language: English
• Published: 2017
• Subjects: RD701 Orthopedics
• Online Access: http://irep.iium.edu.my/57853/21/57853.pdf; http://irep.iium.edu.my/57853/; http://www.apoatrauma.com/eposters.html
• Institution: Universiti Islam Antarabangsa Malaysia

INTRODUCTION: The prevalence of implant associated with infection remains high. The management of this incidence poses a major challenge in orthopaedic field since these scenarios are chronic and biofilm based. Colonization of bacterial and biofilm adhesion on implant may lead to infection at underlying bone and surrounding tissues. Surgically device removal and addition with prolonged used of antimicrobial theraphy are leads to patient suffering, substantial morbidity and even mortality. Hence, to overcome this, alternative treatment strategy using orthopaedic metallic implant coated with 3% silver was introduced. The 3% silver composite exhibits good bactericidal properties. It inhibits biofilm formation particularly, at the adhesion stage of the relevant bacteria. Hence, the aim of this study is to investigate the potential effect of silver coated orthopaedic metal implant against biofilm-producing Staphylococcus aureus through in vitro experimental setting. METHODS: The antibacterial property of orthopaedic metal implants was investigated using Staphylococcus aureus ATCC 25923. The sterile nutrient agar media was prepared. The bacterial colony then was smeared on the surface of the agar media. The coated six holes mini plate and non coated six holes plate which served as control were placed on the surface of agar media. The samples then were incubated for culture under aerobic condition with temperature 37ºC for 24 hours and continued incubated up to 48 hours for assessment. In this study, the antibacterial effect was evaluated based on the diameter of inhibition zone using in situ inhibitory test. RESULTS: The in situ test showed that the colony was noted after 24 hours of incubation. After 48 hours of incubation, the inhibition zone was noted and the number colony did not increase compared to control sample. DISCUSSIONS: Considering the prolonged used of antibiotic as theraphy which leads to resistance, silver coating on orthopaedic implant become very attractive alternative as antibacterial agent. According to Chen et al., (2006) silver is the well known agent compared to other dopants as antibacterial since its character not only can inhibit the bacterial adhesion with broad antibacterial spectrum, but also consists long lasting antibacterial effect. This was proved from the outcome of this study which revealed positive response of the silver as antibacterial agent. The experiment works is still ongoing. CONCLUSION: This result suggested that test material of orthopaedic metal implant coated with silver composite has the intended potential as antibacterial and provide protection against medical device-related infection.