Comparison of purification methods to purify recombinant bromelain from Escherichia coli BL21-A1 = Perbandingan kaedah penulenan rekombinan bromelain daripada Escherichia coli BL21-A1

• Main Authors: Mohd Arshad, Zatul Iffah, Amid, Azura, Yusof, Faridah, Sulaiman, Siti Zubaidah, Abdul Mudalip, Siti Kholijah, Che Man, Rohaida, Md Shaarani, Shalyda
• Format: Article
• Language: English; English
• Published: Faculty of Science and Technology, Universiti Kebangsaan Malaysia 2017
• Subjects: TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/58456/1/58456_Comparison%20of%20purification%20methods.pdf; http://irep.iium.edu.my/58456/2/58456_Comparison%20of%20purification%20methods_SCOPUS.pdf; http://irep.iium.edu.my/58456/; http://www.ukm.my/mjas/v21_n4/pdf/ZatulIffah_21_4_23.pdf
• Institution: Universiti Islam Antarabangsa Malaysia

Recombinant bromelain is a cysteine protease that can be exploited for its protease activity in food and pharmaceutical applications. The aim of this study was to compare different purification methods aqueous two-phase system (ATPS), ammonium sulphate precipitation, ion exchange, affinity, and ge...