Antihyperglycemic activities of protein containing adiponectin from abdominal adipose tissues in the streptozotocin-induced hyperglycemic rats

• Main Authors: Azahari, Nuraniza, Khattak, Muhammad Muzaffar Ali Khan, Mohd Shukri, Nor Azwani
• Format: Proceeding Paper
• Language: English
• Published: 2017
• Subjects: TX341 Nutrition. Foods and food supply
• Online Access: http://irep.iium.edu.my/61731/14/61731_Antihyperglycemic%20Activities%20of%20Protein%20Containing%20_complete.pdf; http://irep.iium.edu.my/61731/; http://www.maso.org.my/index.php?links
• Institution: Universiti Islam Antarabangsa Malaysia

Research Problem/Objectives: White adipose tissue is not just an energy storage depot but now it is recognized to be an active organ in energy homeostasis and other physiological functions. It is known to secrete a variety of bioactive proteins known as adipokines. Among these adipokines, adiponectin is reported to have antidiabetic properties which functions as an insulin sensitizer. The present study was performed to evaluate the effects of protein containing adiponectin from abdominal adipose tissues namely chicken, beef & lamb or insulin alone on blood glucose, blood lipid and histological changes in pancreas of streptozotocin-induced hyperglycemic rats. Materials and Method: In this study, thirty male adult Sprague Dawley rats (Rattus norvegicus) weighing 150-250 grams (8-10 weeks) were randomly divided into five experimental groups A, B, C, D and E. Hyperglycemia was induced by a single intraperitoneal injection of streptozotocin (STZ) at rate of 70 mg/kg body weight. Seven days after, the confirmation of hyperglycemia assessed by glucometer, Groups A, B and C were treated with 30 mg/kg body weight of protein containing adiponectin from chicken, beef and lamb adipocytes. Group D was treated with 2 IU/kg body weight of insulin. Groups A, B, and C served as hyperglycemia and group E was a normal control and received 1 ml/kg body weight buffer. After 14 days of treatment, the animals were sacrificed, the blood was analysed and the pancreas was processed for histological changes. The data were analyzed by one-way ANOVA and the differences in the means were ascertained by using Tukey’s post-hoc test at 95% confidence interval (p<0.05) with the use of SPSS statistical software Results and Discussion: The protein from the aforementioned sources reduced blood glucose and lipids concentration. This hypoglycemic and hypolipidemic effect may be due to the presence of adiponectin in the protein. The present study also demonstrated that these protein sources protects the pancreas against STZ-induced hyperglycemic and had resulted in islets regeneration of pancreatic beta cells. Conclusion: Protein sources from adipocytes improve hyperglycaemia and hyperlipidaemia in STZ-induced hyperglycemic rats. Furthermore, it regenerate damage pancreatic β-cells of the rats.