Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene

Epigenetic DNA methylation and Schizophrenia The evidence of Disrupted in Schizophrenia 1 (DISC1) gene role in schizophrenia came through functional and animal studies. However the exact genetic basis is yet established. As meta-analyses findings have denied DISC1 variants as the genetic basis...

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Main Authors: A.Talib, Norlelawati, Ku Zaifah, Norsidah, Abdullah, Kartini, Abd. Rahim, Nour El Huda
Format: Monograph
Language:English
Published: 2018
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Online Access:http://irep.iium.edu.my/63787/1/PROJECT%20REPORT%20PROFILE.pdf
http://irep.iium.edu.my/63787/
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Institution: Universiti Islam Antarabangsa Malaysia
Language: English
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spelling my.iium.irep.637872019-01-11T03:03:00Z http://irep.iium.edu.my/63787/ Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene A.Talib, Norlelawati Ku Zaifah, Norsidah Abdullah, Kartini Abd. Rahim, Nour El Huda RM Therapeutics. Pharmacology RZ400 Mental healing Epigenetic DNA methylation and Schizophrenia The evidence of Disrupted in Schizophrenia 1 (DISC1) gene role in schizophrenia came through functional and animal studies. However the exact genetic basis is yet established. As meta-analyses findings have denied DISC1 variants as the genetic basis, emphasis is now shifted to genetic defect such as copy number and epigenetic which have not been well explored. The project explored the epigenetic regulation of DISC1 gene and other neurodevelopmental and neurotransmitter genes that were strongly advocated as candidate genes of schizophrenia. A comparative cross sectional study consisted of 110 schizophrenia and 122 healthy control were recruited. Method for DNA methylation quantitation using high resolution melting (HRM) and MethylLight assays were established and used to quantify the methylation percentage. The optimized HRM assay for DISC1clearly demonstrated the serial methylated percentage differentiation of 0%, 25%, 50%, 75% and 100% accordingly. However, we found DISC1 methylation has no relationship with schizophrenia (p >0.05). With similar approach, COMT and RELN DNA methylation were investigated. We found, a significantly higher level of methylation of the RELN and COMT promoter in patients with schizophrenia compared to controls (p=0.005) and reduced RELN but not COMT expression in the methylated group as compared to the non-methylated group. The findings suggest possibility of DNA methylation of RELN and COMT gene effects in the pathogenesis of schizophrenia. 2018-05 Monograph NonPeerReviewed application/pdf en http://irep.iium.edu.my/63787/1/PROJECT%20REPORT%20PROFILE.pdf A.Talib, Norlelawati and Ku Zaifah, Norsidah and Abdullah, Kartini and Abd. Rahim, Nour El Huda (2018) Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene. Project Report. UNSPECIFIED. (Unpublished)
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic RM Therapeutics. Pharmacology
RZ400 Mental healing
spellingShingle RM Therapeutics. Pharmacology
RZ400 Mental healing
A.Talib, Norlelawati
Ku Zaifah, Norsidah
Abdullah, Kartini
Abd. Rahim, Nour El Huda
Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene
description Epigenetic DNA methylation and Schizophrenia The evidence of Disrupted in Schizophrenia 1 (DISC1) gene role in schizophrenia came through functional and animal studies. However the exact genetic basis is yet established. As meta-analyses findings have denied DISC1 variants as the genetic basis, emphasis is now shifted to genetic defect such as copy number and epigenetic which have not been well explored. The project explored the epigenetic regulation of DISC1 gene and other neurodevelopmental and neurotransmitter genes that were strongly advocated as candidate genes of schizophrenia. A comparative cross sectional study consisted of 110 schizophrenia and 122 healthy control were recruited. Method for DNA methylation quantitation using high resolution melting (HRM) and MethylLight assays were established and used to quantify the methylation percentage. The optimized HRM assay for DISC1clearly demonstrated the serial methylated percentage differentiation of 0%, 25%, 50%, 75% and 100% accordingly. However, we found DISC1 methylation has no relationship with schizophrenia (p >0.05). With similar approach, COMT and RELN DNA methylation were investigated. We found, a significantly higher level of methylation of the RELN and COMT promoter in patients with schizophrenia compared to controls (p=0.005) and reduced RELN but not COMT expression in the methylated group as compared to the non-methylated group. The findings suggest possibility of DNA methylation of RELN and COMT gene effects in the pathogenesis of schizophrenia.
format Monograph
author A.Talib, Norlelawati
Ku Zaifah, Norsidah
Abdullah, Kartini
Abd. Rahim, Nour El Huda
author_facet A.Talib, Norlelawati
Ku Zaifah, Norsidah
Abdullah, Kartini
Abd. Rahim, Nour El Huda
author_sort A.Talib, Norlelawati
title Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene
title_short Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene
title_full Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene
title_fullStr Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene
title_full_unstemmed Epigenetics of schizophrenia: A focus on the DNA methylation mechanism and expression of DISC1 gene
title_sort epigenetics of schizophrenia: a focus on the dna methylation mechanism and expression of disc1 gene
publishDate 2018
url http://irep.iium.edu.my/63787/1/PROJECT%20REPORT%20PROFILE.pdf
http://irep.iium.edu.my/63787/
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