Development of an amperometric glucose biosensor based on the immobilization of glucose oxidase on the Se-MCM-41 mesoporous composite

• Main Authors: Yusan, Sabriye, M. Rahman, Mokhlesur, Mohamad, Nasir A.N., ,, Tengku M. Arrif, Abdul Latif, Ahmad Zubaidi, Md Aris, Mohd Aznan, Wan Nik, Wan Sani
• Format: Article
• Language: English; English; English
• Published: Hindawi 2018
• Subjects: R Medicine (General); RM Therapeutics. Pharmacology; RS Pharmacy and materia medica; RZ Other systems of medicine
• Online Access: http://irep.iium.edu.my/63788/1/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase.pdf; http://irep.iium.edu.my/63788/2/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_SCOPUS.pdf; http://irep.iium.edu.my/63788/3/63788_Development%20of%20an%20amperometric%20glucose%20biosensor%20based%20on%20the%20immobilization%20of%20glucose%20oxidase_WoS.pdf; http://irep.iium.edu.my/63788/; https://doi.org/10.1155/2018/2687341
• Institution: Universiti Islam Antarabangsa Malaysia

A new bioenzymatic glucose biosensor for selective and sensitive detection of glucose was developed by the immobilization of glucose oxidase (GOD) onto selenium nanoparticle-mesoporous silica composite (MCM-41) matrix and then prepared as a carbon paste electrode (CPE). Cyclic voltammetry was employed to probe the catalytic behavior of the biosensor. A linear calibrationplotisobtainedoverawideconcentrationrangeofglucosefrom1×10−5 to2×10−3M.Underoptimalconditions,the biosensor exhibits high sensitivity (0.34µA·mM−1), low detection limit (1×10−4M), high affinity to glucose (Km�0.02mM), and also good reproducibility (R.S.D. 2.8%, n � 10) and a stability of about ten days when stored dry at +4°C. Besides, the effectsofpHvalue,scanrate,mediatoreffectsontheglucosecurrent,andelectroactiveinterferenceofthebiosensorwerealso discussed. As a result, the biosensor exhibited an excellent electrocatalytic response to glucose as well as unique stability and reproducibility.