Improved skin wound healing activity of insulin cream as evidenced from the morphological evaluation in guinea pigs

• Main Authors: Mohamed, Nur-Aliana Hidayah, Mokhtar, Rafidah Hanim, Al-Ani, Imad Matloub Dally, Ayob, Azizi, Misran, Misni
• Format: Article
• Language: English; English
• Published: Universitas Indonesia 2018
• Subjects: QM Human anatomy; QP Physiology; RB Pathology; RL Dermatology
• Online Access: http://irep.iium.edu.my/66214/1/66214_Improved%20Skin%20Wound%20Healing%20Activity%20of%20Insulin%20Cream_article.pdf; http://irep.iium.edu.my/66214/7/66214%20Improved%20Skin%20Wound%20Healing%20Activity%20of%20Insulin%20Cream%20as%20Evidenced%20from%20the%20Morphological%20Evaluation%20in%20Guinea%20Pigs_wos.pdf; http://irep.iium.edu.my/66214/; http://journal.ui.ac.id/index.php/health/article/view/9714/pdf
• Institution: Universiti Islam Antarabangsa Malaysia

Background: There is no histological study evaluating the effects of insulin-containing cream on skin injury. The goal of this study was to examine the effects of insulin-containing creams on wound healing. Methods: Creams consisting of nine parts of oil and one part of aqueous phase (9:1) mixed with 1.5 mL human insulin were prepared. Eighteen male guinea pigs were divided into three groups; the control (9:1 C) group received cream without insulin. The experimental groups received Humulin N (9:1 N) and Humulin R (9:1 R) respectively. A 1 cm2 wound of 1-2 cm thickness was created in the skin. Each animal received 0.5 g of the respective creams which was topically applied once a day for 14 days. The progress of wound healing was monitored daily. Skin tissues were excised at the 14th days from the wound sites and processed for light microscopy. Results: Skin wound treated with the long acting insulin Humulin N had an accelerated wound healing process with restoration of vascular network, increased collagen deposition and early complete wound remodeling. Conclusion: Insulin cream with long acting mechanism facilitates in normalizing cell permeability, promoting vascularization, reducing exudation and stimulate proliferation of cells. These properties render insulin cream suitable for expediting wound healing.