Lutein production by Scenedesmus Obliquus cultivated in stirred-tank bioreactor

Microalgae were known to have higher lutein content, easy to grow, and abundantly live in the wet environment. Optimization of lutein from microalgae, Scenedesmus obliquus is critical for commercialization of lutein. This research focused on lutein production from locally isolated S. obliquus which...

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Main Authors: Mustapa, Mokhzanni, Raus, Raha Bt. Ahmad
Format: Book
Language:English
Published: IIUM Press, International Islamic University Malaysia 2017
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Online Access:http://irep.iium.edu.my/74954/1/74954_Lutein%20production%20by%20Scenedesmus%20Obliquus%20cultivated%20in%20stirred-tank%20bioreactor.pdf
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spelling my.iium.irep.749542022-01-07T07:44:55Z http://irep.iium.edu.my/74954/ Lutein production by Scenedesmus Obliquus cultivated in stirred-tank bioreactor Mustapa, Mokhzanni Raus, Raha Bt. Ahmad TP Chemical technology Microalgae were known to have higher lutein content, easy to grow, and abundantly live in the wet environment. Optimization of lutein from microalgae, Scenedesmus obliquus is critical for commercialization of lutein. This research focused on lutein production from locally isolated S. obliquus which was cultivated in 2.5 L stirred tank bioreactor. Five bioreactors parameters which were stirrer speed, air flow rate, light exposure period, starting inoculums size, and temperature were investigated for their effects towards lutein content as well as biomass of microalgae. Initial lutein amount detected was 41.4 mg/g with biomass density of 0.456 g/l at 25°C, 0.5 liter/minute of aeration flow rate, 100 rpm, and 12 hours light exposure. The effect of five bioreactor parameters was analyzed by using Main Effect Pareto Charts, followed by ANOVA analysis. Stirrer speed and starting inoculums size have been identified as noise and removed from further optimization. FaceCentred Central Composite Design (FCCCD) under Response Surface Method (RSM) was used as design tool to optimize temperature, lighting period and air flow rate. Based on the 20 runs of FCCCD, microalgae biomass density observed was between 0.285 g/l (min) to 0.717 g/l (max), while the lutein content was between 12.231 mg/g (min) to 91.099 mg/g (max). IIUM Press, International Islamic University Malaysia 2017 Book PeerReviewed application/pdf en http://irep.iium.edu.my/74954/1/74954_Lutein%20production%20by%20Scenedesmus%20Obliquus%20cultivated%20in%20stirred-tank%20bioreactor.pdf Mustapa, Mokhzanni and Raus, Raha Bt. Ahmad (2017) Lutein production by Scenedesmus Obliquus cultivated in stirred-tank bioreactor. IIUM Press, International Islamic University Malaysia, Kuala Lumpur , Malaysia. ISBN 978-967-418-491-9
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Mustapa, Mokhzanni
Raus, Raha Bt. Ahmad
Lutein production by Scenedesmus Obliquus cultivated in stirred-tank bioreactor
description Microalgae were known to have higher lutein content, easy to grow, and abundantly live in the wet environment. Optimization of lutein from microalgae, Scenedesmus obliquus is critical for commercialization of lutein. This research focused on lutein production from locally isolated S. obliquus which was cultivated in 2.5 L stirred tank bioreactor. Five bioreactors parameters which were stirrer speed, air flow rate, light exposure period, starting inoculums size, and temperature were investigated for their effects towards lutein content as well as biomass of microalgae. Initial lutein amount detected was 41.4 mg/g with biomass density of 0.456 g/l at 25°C, 0.5 liter/minute of aeration flow rate, 100 rpm, and 12 hours light exposure. The effect of five bioreactor parameters was analyzed by using Main Effect Pareto Charts, followed by ANOVA analysis. Stirrer speed and starting inoculums size have been identified as noise and removed from further optimization. FaceCentred Central Composite Design (FCCCD) under Response Surface Method (RSM) was used as design tool to optimize temperature, lighting period and air flow rate. Based on the 20 runs of FCCCD, microalgae biomass density observed was between 0.285 g/l (min) to 0.717 g/l (max), while the lutein content was between 12.231 mg/g (min) to 91.099 mg/g (max).
format Book
author Mustapa, Mokhzanni
Raus, Raha Bt. Ahmad
author_facet Mustapa, Mokhzanni
Raus, Raha Bt. Ahmad
author_sort Mustapa, Mokhzanni
title Lutein production by Scenedesmus Obliquus cultivated in stirred-tank bioreactor
title_short Lutein production by Scenedesmus Obliquus cultivated in stirred-tank bioreactor
title_full Lutein production by Scenedesmus Obliquus cultivated in stirred-tank bioreactor
title_fullStr Lutein production by Scenedesmus Obliquus cultivated in stirred-tank bioreactor
title_full_unstemmed Lutein production by Scenedesmus Obliquus cultivated in stirred-tank bioreactor
title_sort lutein production by scenedesmus obliquus cultivated in stirred-tank bioreactor
publisher IIUM Press, International Islamic University Malaysia
publishDate 2017
url http://irep.iium.edu.my/74954/1/74954_Lutein%20production%20by%20Scenedesmus%20Obliquus%20cultivated%20in%20stirred-tank%20bioreactor.pdf
http://irep.iium.edu.my/74954/
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