GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata

Cyperaceae species have an intrinsic value as a source of active elements with biological activity from the family of monocotyledonous known as sedges. Sedges grow in all types of soils associated with wetlands or poor soils. The aim of this present study is to evaluate the content of phenolic comp...

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Main Authors: Othman, Rashidi, Ramya, Razanah, Mohd Hassan, Norazian, Kammona, Suhair
Format: Article
Language:English
Published: SET PUBLISHER 2020
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Online Access:http://irep.iium.edu.my/78490/7/78490_GCTOF-MS%20and%20HPLC%20Identification%20of%20Phenolic%20Compounds.pdf
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spelling my.iium.irep.784902020-11-17T04:41:49Z http://irep.iium.edu.my/78490/ GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata Othman, Rashidi Ramya, Razanah Mohd Hassan, Norazian Kammona, Suhair GE Environmental Sciences RS Pharmacy and materia medica SB Plant culture TP248.13 Biotechnology TP934 Paints, pigments, varnishes, etc Cyperaceae species have an intrinsic value as a source of active elements with biological activity from the family of monocotyledonous known as sedges. Sedges grow in all types of soils associated with wetlands or poor soils. The aim of this present study is to evaluate the content of phenolic compounds by qualitative and quantitive analysis on Lepironia articulata. Dried leaves of L. articulata were successfully extracted by using water extraction then separated with different solvent polarities; petroleum ether, ethyl acetate and butanol fractions before being analysed using GCTOF-MS, microplate reader and HPLC. The result from the GCTOF-MS analysis of fractional extracts showed that 48 compounds were found in petroleum ether, ethyl acetate and butanol extracts. From those extracts, only six phenolic compounds were identified in ethyl acetate and butanol extracts which were 2-Methoxy-4-vinylphenol, Phenol, 2,4- bis(1,1-dimethylethyl)-, 4-Hydroxybenzaldehyde, Catechol, Phenol, 2-methoxy- and Vanillin. The total phenolic content was found to be 984.63 ± 5.96 μg GAE/g DW in L. articulata. Quantitative analysis of individual phenolic acid by HPLC showed the predominant amount of Vanillic acid (0.48 ± 0.00 μg/g DW) in ethyl acetate while 4-Hydroxybenzoic acid and Caffeic acid, both of which were 0.12 ± 0.00 μg/g DW in butanol extracts. In the present study, the plant extracts demonstrated the highest phenolic compound detected in ethyl acetate and butanol. SET PUBLISHER 2020-02-04 Article PeerReviewed application/pdf en http://irep.iium.edu.my/78490/7/78490_GCTOF-MS%20and%20HPLC%20Identification%20of%20Phenolic%20Compounds.pdf Othman, Rashidi and Ramya, Razanah and Mohd Hassan, Norazian and Kammona, Suhair (2020) GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata. Journal of Pharmacy and Nutrition Sciences, 10 (1). pp. 1-6. ISSN 2223-3806 E-ISSN 1927-5951
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic GE Environmental Sciences
RS Pharmacy and materia medica
SB Plant culture
TP248.13 Biotechnology
TP934 Paints, pigments, varnishes, etc
spellingShingle GE Environmental Sciences
RS Pharmacy and materia medica
SB Plant culture
TP248.13 Biotechnology
TP934 Paints, pigments, varnishes, etc
Othman, Rashidi
Ramya, Razanah
Mohd Hassan, Norazian
Kammona, Suhair
GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata
description Cyperaceae species have an intrinsic value as a source of active elements with biological activity from the family of monocotyledonous known as sedges. Sedges grow in all types of soils associated with wetlands or poor soils. The aim of this present study is to evaluate the content of phenolic compounds by qualitative and quantitive analysis on Lepironia articulata. Dried leaves of L. articulata were successfully extracted by using water extraction then separated with different solvent polarities; petroleum ether, ethyl acetate and butanol fractions before being analysed using GCTOF-MS, microplate reader and HPLC. The result from the GCTOF-MS analysis of fractional extracts showed that 48 compounds were found in petroleum ether, ethyl acetate and butanol extracts. From those extracts, only six phenolic compounds were identified in ethyl acetate and butanol extracts which were 2-Methoxy-4-vinylphenol, Phenol, 2,4- bis(1,1-dimethylethyl)-, 4-Hydroxybenzaldehyde, Catechol, Phenol, 2-methoxy- and Vanillin. The total phenolic content was found to be 984.63 ± 5.96 μg GAE/g DW in L. articulata. Quantitative analysis of individual phenolic acid by HPLC showed the predominant amount of Vanillic acid (0.48 ± 0.00 μg/g DW) in ethyl acetate while 4-Hydroxybenzoic acid and Caffeic acid, both of which were 0.12 ± 0.00 μg/g DW in butanol extracts. In the present study, the plant extracts demonstrated the highest phenolic compound detected in ethyl acetate and butanol.
format Article
author Othman, Rashidi
Ramya, Razanah
Mohd Hassan, Norazian
Kammona, Suhair
author_facet Othman, Rashidi
Ramya, Razanah
Mohd Hassan, Norazian
Kammona, Suhair
author_sort Othman, Rashidi
title GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata
title_short GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata
title_full GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata
title_fullStr GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata
title_full_unstemmed GCTOF-MS and HPLC Identification of Phenolic Compounds with Different Fractional Extracts of Lepironia articulata
title_sort gctof-ms and hplc identification of phenolic compounds with different fractional extracts of lepironia articulata
publisher SET PUBLISHER
publishDate 2020
url http://irep.iium.edu.my/78490/7/78490_GCTOF-MS%20and%20HPLC%20Identification%20of%20Phenolic%20Compounds.pdf
http://irep.iium.edu.my/78490/
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