Development of CRISPR-Cas9-mediated site-directed mutagenesis of ECE1 gene (candidalysin) in Candida albicans

• Main Authors: Engku Nasrullah Satiman, Engku Anis Fariha, Kaderi, Mohd Arifin, Ramzi, Ahmad Bazli, Arzmi, Mohd Hafiz
• Format: Conference or Workshop Item
• Language: English; English
• Published: 2020
• Subjects: QR Microbiology; RK Dentistry
• Online Access: http://irep.iium.edu.my/80883/1/Proceeding%20ARCSB%202020%20Engku%20Anis.pdf; http://irep.iium.edu.my/80883/2/ARCSB%20POSTER%20EAFENS%20TO%20PRINT%20pdf.pdf; http://irep.iium.edu.my/80883/
• Institution: Universiti Islam Antarabangsa Malaysia

CRISPR-Cas9 has been extensively used in microbial editing considering its specificity and versatility. Cumbersome editing in C. albicans due to its aberrant chromosomal ploidy and lock of meiosis can be potentially solved using CRISPR-Cas9. In this study, the third KR (lysine-arginine) motif on the...