Marantodes pumilum (Blume) Kuntze (Kacip Fatimah) stimulates uterine contraction in rats in post-partum period

thnopharmacological relevance: Marantodes pumilum (Blume) Kuntze has traditionally been used to firm the uterus after delivery, however scientific evidences behind this claim is still lacking. Aims of study: To demonstrate Marantodes pumilum leaves aqueous extract (MPE) has an effect on uterine con...

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Main Authors: Wan Omar, Wan Fatein Nabeila, Giribabu, Nelli, Karim, Kamarulzaman, Salleh, Naguib
Format: Article
Language:English
Published: Elsevier 2019
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Online Access:http://irep.iium.edu.my/81635/1/wanomar2019.pdf
http://irep.iium.edu.my/81635/
https://www.journals.elsevier.com/journal-of-ethnopharmacology
https://doi.org/10.1016/j.jep.2019.112175
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spelling my.iium.irep.816352020-08-03T04:35:02Z http://irep.iium.edu.my/81635/ Marantodes pumilum (Blume) Kuntze (Kacip Fatimah) stimulates uterine contraction in rats in post-partum period Wan Omar, Wan Fatein Nabeila Giribabu, Nelli Karim, Kamarulzaman Salleh, Naguib QP Physiology thnopharmacological relevance: Marantodes pumilum (Blume) Kuntze has traditionally been used to firm the uterus after delivery, however scientific evidences behind this claim is still lacking. Aims of study: To demonstrate Marantodes pumilum leaves aqueous extract (MPE) has an effect on uterine contraction after delivery and to elucidate the molecular mechanisms involved. Methods: Day-1 post-delivery female rats were given MPE (100, 250 and 500 mg/kg/day) orally for seven consecutive days. A day after the last treatment (day-8), rats were sacrificed and uteri were harvested and subjected for ex-vivo contraction study using organ bath followed by protein expression and distribution study by Western blotting and immunohistochemistry techniques, respectively. The proteins of interest include calmo- dulin-CaM, myosin light chain kinase-MLCK, sarcoplasmic reticulum Ca2+-ATPase (SERCA), G-protein α and β (Gα and Gβ), inositol-triphosphate 3-kinase (IP3K), oxytocin receptor-OTR, prostaglandin (PGF)2α receptor- PGFR, muscarinic receptor-MAChR and estrogen receptor (ER) isoforms α and β. Levels of estradiol and pro- gesterone in serum were determined by enzyme-linked immunoassay (ELISA). Results: Ex-vivo contraction study revealed the force of uterine contraction increased with increasing doses of MPE. In addition, expression of CaM, MLCK, SERCA, Gα, Gβ, IP3K, OTR, PGF2α, MAChR, Erα and ERβ in the uterus increased with increasing doses of MPE. Serum analysis indicate that estradiol levels decreased while progesterone levels remained low at day-8 post-partum in rats receiving 250 and 500 mg/kg/day MPE. Conclusions: These findings support the claims that MPE help to firm the uterus and pave the way for its use as a uterotonic agent after delivery. Elsevier 2019-12-05 Article PeerReviewed application/pdf en http://irep.iium.edu.my/81635/1/wanomar2019.pdf Wan Omar, Wan Fatein Nabeila and Giribabu, Nelli and Karim, Kamarulzaman and Salleh, Naguib (2019) Marantodes pumilum (Blume) Kuntze (Kacip Fatimah) stimulates uterine contraction in rats in post-partum period. Journal of Ethnopharmacology, 245. pp. 1-9. ISSN 0378-8741 https://www.journals.elsevier.com/journal-of-ethnopharmacology https://doi.org/10.1016/j.jep.2019.112175
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic QP Physiology
spellingShingle QP Physiology
Wan Omar, Wan Fatein Nabeila
Giribabu, Nelli
Karim, Kamarulzaman
Salleh, Naguib
Marantodes pumilum (Blume) Kuntze (Kacip Fatimah) stimulates uterine contraction in rats in post-partum period
description thnopharmacological relevance: Marantodes pumilum (Blume) Kuntze has traditionally been used to firm the uterus after delivery, however scientific evidences behind this claim is still lacking. Aims of study: To demonstrate Marantodes pumilum leaves aqueous extract (MPE) has an effect on uterine contraction after delivery and to elucidate the molecular mechanisms involved. Methods: Day-1 post-delivery female rats were given MPE (100, 250 and 500 mg/kg/day) orally for seven consecutive days. A day after the last treatment (day-8), rats were sacrificed and uteri were harvested and subjected for ex-vivo contraction study using organ bath followed by protein expression and distribution study by Western blotting and immunohistochemistry techniques, respectively. The proteins of interest include calmo- dulin-CaM, myosin light chain kinase-MLCK, sarcoplasmic reticulum Ca2+-ATPase (SERCA), G-protein α and β (Gα and Gβ), inositol-triphosphate 3-kinase (IP3K), oxytocin receptor-OTR, prostaglandin (PGF)2α receptor- PGFR, muscarinic receptor-MAChR and estrogen receptor (ER) isoforms α and β. Levels of estradiol and pro- gesterone in serum were determined by enzyme-linked immunoassay (ELISA). Results: Ex-vivo contraction study revealed the force of uterine contraction increased with increasing doses of MPE. In addition, expression of CaM, MLCK, SERCA, Gα, Gβ, IP3K, OTR, PGF2α, MAChR, Erα and ERβ in the uterus increased with increasing doses of MPE. Serum analysis indicate that estradiol levels decreased while progesterone levels remained low at day-8 post-partum in rats receiving 250 and 500 mg/kg/day MPE. Conclusions: These findings support the claims that MPE help to firm the uterus and pave the way for its use as a uterotonic agent after delivery.
format Article
author Wan Omar, Wan Fatein Nabeila
Giribabu, Nelli
Karim, Kamarulzaman
Salleh, Naguib
author_facet Wan Omar, Wan Fatein Nabeila
Giribabu, Nelli
Karim, Kamarulzaman
Salleh, Naguib
author_sort Wan Omar, Wan Fatein Nabeila
title Marantodes pumilum (Blume) Kuntze (Kacip Fatimah) stimulates uterine contraction in rats in post-partum period
title_short Marantodes pumilum (Blume) Kuntze (Kacip Fatimah) stimulates uterine contraction in rats in post-partum period
title_full Marantodes pumilum (Blume) Kuntze (Kacip Fatimah) stimulates uterine contraction in rats in post-partum period
title_fullStr Marantodes pumilum (Blume) Kuntze (Kacip Fatimah) stimulates uterine contraction in rats in post-partum period
title_full_unstemmed Marantodes pumilum (Blume) Kuntze (Kacip Fatimah) stimulates uterine contraction in rats in post-partum period
title_sort marantodes pumilum (blume) kuntze (kacip fatimah) stimulates uterine contraction in rats in post-partum period
publisher Elsevier
publishDate 2019
url http://irep.iium.edu.my/81635/1/wanomar2019.pdf
http://irep.iium.edu.my/81635/
https://www.journals.elsevier.com/journal-of-ethnopharmacology
https://doi.org/10.1016/j.jep.2019.112175
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