Anti-cancer effects of Eurycoma longifolia jack dichloromethane root extract on nasopharyngeal carcinoma cell line

• Main Authors: Kajahmohideen, Nur Haseena, Razi, Siti NurSyafiqah, Faisal, Ghasak Ghazi, Ashour, Abdelkader Elbadawy Abbas
• Format: Conference or Workshop Item
• Language: English; English; English
• Published: 2020
• Subjects: RB Pathology
• Online Access: http://irep.iium.edu.my/82984/3/Poster%20presentation%20for%20ICOMOI2020.pdf; http://irep.iium.edu.my/82984/1/icomoi-2020-abstract-book%20dr%20ghasak.pdf; http://irep.iium.edu.my/82984/2/E-poster%20Assoc.Prof.Dr.Ghasak%20Ghazi%20Faisal%20certificate.pdf; http://irep.iium.edu.my/82984/; https://mysomoi.org/meetings/icomoi-2020/speakers-and-program-details/
• Institution: Universiti Islam Antarabangsa Malaysia

Eurycoma longifolia jack (Tongkat Ali) root extract is well known to contain bioactive compounds that exhibit many properties including cytotoxic activity against cancer cells. The purpose of this study was to assess the cytotoxic activity of dichloromethane DCM root extract against nasopharyngeal cancer cell line (ORL-115). E. longifolia root was extracted using DCM solvent by Soxhlet method. MTS assay was used to evaluate the cytotoxic effect of the root extract against ORL-115 cell line for three different incubation times which were 24-hour, 48-hour, and 72-hour. Results showed that DCM extracts exert cytotoxic activity against ORL-115 cell line. The IC50 of DCM extract were 678.87 µg/ml, 136.71 µg/ml, 73.72 µg/ml for 24-hour, 48-hour and 72-hour incubation period, respectively. The cytotoxic activity increased as the incubation time increased. Our results demonstrate that DCM root exerted cytotoxic activity against the nasopharyngeal carcinoma cell line. The cytotoxic activity of the extract was dose dependent and time dependent.