Discovery of cold-active protease from psychrophilic bacteria isolated from Antarctic region for bio-prospecting
The Antarctic region is a new frontier as natural sources for bio-prospecting purposes. Its extreme cold temperature may provide unique enzyme characteristics that have valuable potential for industrial and biotechnological applications. This study was designed to discover proteases that are activ...
Saved in:
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English English |
Published: |
Malaysian Society of Applied Biology
2020
|
Subjects: | |
Online Access: | http://irep.iium.edu.my/84087/1/84087_Discovery%20of%20cold-active%20protease.pdf http://irep.iium.edu.my/84087/2/84087_Discovery%20of%20cold-active%20protease_SCOPUS.pdf http://irep.iium.edu.my/84087/ http://www.mabjournal.com/images/49_1_June_2020/49_01_06.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Institution: | Universiti Islam Antarabangsa Malaysia |
Language: | English English |
id |
my.iium.irep.84087 |
---|---|
record_format |
dspace |
spelling |
my.iium.irep.840872021-03-02T07:45:07Z http://irep.iium.edu.my/84087/ Discovery of cold-active protease from psychrophilic bacteria isolated from Antarctic region for bio-prospecting Abd Latip, Muhammad Asyraf Alias, Siti Aisyah Smykla, Jerzy Yusof, Faridah Mohamad, Mohd Azrul Naim Nordin, Noor Faizul Hadry Q Science (General) TP248.13 Biotechnology The Antarctic region is a new frontier as natural sources for bio-prospecting purposes. Its extreme cold temperature may provide unique enzyme characteristics that have valuable potential for industrial and biotechnological applications. This study was designed to discover proteases that are activate and can work at very low temperatures. Soil samples from the Antarctic region were screened for protease activity on skim milk agar at 4°C. Bacteria that showed clear halo zone around the colonies were selected and identified through 16S rDNA sequencing. Out of 35 bacteria, 10 bacteria that showed rapid halo zone formation were selected and further analyzed by enzymatic assay. By using azocasein as a substrate, the reaction was measured using spectrophotometer at OD340 nm. Based on the 16S rDNA sequence, phylogenetic analysis showed that 88% of the bacteria producing protease were from Pseudomonas sp., 9% from Arthrobacter sp. and 3% from Paenibacillus sp. For enzymatic assay analysis, sample SC8 showed the highest protease activity compared to other 10 samples. This preliminary study successfully demonstrated cold active protease producers that can be further investigated for bioprospecting. In future, purification and characterization of this enzyme is required in order to optimize the enzyme activity. Malaysian Society of Applied Biology 2020-06-30 Article PeerReviewed application/pdf en http://irep.iium.edu.my/84087/1/84087_Discovery%20of%20cold-active%20protease.pdf application/pdf en http://irep.iium.edu.my/84087/2/84087_Discovery%20of%20cold-active%20protease_SCOPUS.pdf Abd Latip, Muhammad Asyraf and Alias, Siti Aisyah and Smykla, Jerzy and Yusof, Faridah and Mohamad, Mohd Azrul Naim and Nordin, Noor Faizul Hadry (2020) Discovery of cold-active protease from psychrophilic bacteria isolated from Antarctic region for bio-prospecting. Malaysian Applied Biology, 49 (1). pp. 55-60. ISSN 0126-8643 http://www.mabjournal.com/images/49_1_June_2020/49_01_06.pdf |
institution |
Universiti Islam Antarabangsa Malaysia |
building |
IIUM Library |
collection |
Institutional Repository |
continent |
Asia |
country |
Malaysia |
content_provider |
International Islamic University Malaysia |
content_source |
IIUM Repository (IREP) |
url_provider |
http://irep.iium.edu.my/ |
language |
English English |
topic |
Q Science (General) TP248.13 Biotechnology |
spellingShingle |
Q Science (General) TP248.13 Biotechnology Abd Latip, Muhammad Asyraf Alias, Siti Aisyah Smykla, Jerzy Yusof, Faridah Mohamad, Mohd Azrul Naim Nordin, Noor Faizul Hadry Discovery of cold-active protease from psychrophilic bacteria isolated from Antarctic region for bio-prospecting |
description |
The Antarctic region is a new frontier as natural sources for bio-prospecting purposes. Its extreme cold temperature may
provide unique enzyme characteristics that have valuable potential for industrial and biotechnological applications. This study
was designed to discover proteases that are activate and can work at very low temperatures. Soil samples from the Antarctic
region were screened for protease activity on skim milk agar at 4°C. Bacteria that showed clear halo zone around the colonies
were selected and identified through 16S rDNA sequencing. Out of 35 bacteria, 10 bacteria that showed rapid halo zone
formation were selected and further analyzed by enzymatic assay. By using azocasein as a substrate, the reaction was measured
using spectrophotometer at OD340 nm. Based on the 16S rDNA sequence, phylogenetic analysis showed that 88% of the
bacteria producing protease were from Pseudomonas sp., 9% from Arthrobacter sp. and 3% from Paenibacillus sp. For
enzymatic assay analysis, sample SC8 showed the highest protease activity compared to other 10 samples. This preliminary
study successfully demonstrated cold active protease producers that can be further investigated for bioprospecting. In future,
purification and characterization of this enzyme is required in order to optimize the enzyme activity. |
format |
Article |
author |
Abd Latip, Muhammad Asyraf Alias, Siti Aisyah Smykla, Jerzy Yusof, Faridah Mohamad, Mohd Azrul Naim Nordin, Noor Faizul Hadry |
author_facet |
Abd Latip, Muhammad Asyraf Alias, Siti Aisyah Smykla, Jerzy Yusof, Faridah Mohamad, Mohd Azrul Naim Nordin, Noor Faizul Hadry |
author_sort |
Abd Latip, Muhammad Asyraf |
title |
Discovery of cold-active protease from psychrophilic bacteria isolated from Antarctic region for bio-prospecting |
title_short |
Discovery of cold-active protease from psychrophilic bacteria isolated from Antarctic region for bio-prospecting |
title_full |
Discovery of cold-active protease from psychrophilic bacteria isolated from Antarctic region for bio-prospecting |
title_fullStr |
Discovery of cold-active protease from psychrophilic bacteria isolated from Antarctic region for bio-prospecting |
title_full_unstemmed |
Discovery of cold-active protease from psychrophilic bacteria isolated from Antarctic region for bio-prospecting |
title_sort |
discovery of cold-active protease from psychrophilic bacteria isolated from antarctic region for bio-prospecting |
publisher |
Malaysian Society of Applied Biology |
publishDate |
2020 |
url |
http://irep.iium.edu.my/84087/1/84087_Discovery%20of%20cold-active%20protease.pdf http://irep.iium.edu.my/84087/2/84087_Discovery%20of%20cold-active%20protease_SCOPUS.pdf http://irep.iium.edu.my/84087/ http://www.mabjournal.com/images/49_1_June_2020/49_01_06.pdf |
_version_ |
1693725784167940096 |