The role of polyamines in beta vulguris (beetroot), zizhipus jujube (jujube) and vitis venefera (raisin) on human lung adenocarcinoma cells (A549) proliferation and apoptosis

• Main Authors: Jamil, Elyna Fatini, Abd Nadzir, Nur Sarah Nabila, Abdul Ghani, Radiah
• Format: Article
• Language: English; English
• Published: Wolters Kluwer Medknow Publications , Wolters Kluwer India Pvt Ltd 2020
• Subjects: RM Therapeutics. Pharmacology
• Online Access: http://irep.iium.edu.my/84765/1/JPharmBioallSci_ICPRP%20ABSTRACTS.pdf; http://irep.iium.edu.my/84765/7/84765%20The%20role%20of%20polyamines%20in%20beta%20vulguris.pdf; http://irep.iium.edu.my/84765/; https://www.jpbsonline.org/article.asp?issn=0975-7406;year=2020;volume=12;issue=6;spage=852;epage=934;aulast=
• Institution: Universiti Islam Antarabangsa Malaysia

Introduction: Polyamines are vital in maintaining human health because they are crucial for cell growth. Excessive intracellular polyamines in diet may promote unwarranted cell proliferation. Objective: This study aimed to investigate polyamines as a chemopreventive agent in selected fruits against human lung adenocarcinoma cells, A549. Materials and Methods: Beta vulgaris (beetroot), Ziziphus jujube (jujube) and Vitis vinifera (raisin) were freeze dried and kept at -80 °C until analysis. The anti-proliferative activity of selected fruits was assessed on A549 cells using MTT assay and the effect on A549 growth was completed via Trypan bluedye exclusion assay. Cell cycle profile and apoptosis were conducted with flow cytometer, while caspase assay was done using microtiter plate-reader. The quantification of intracellular polyamines was done using HPLC and the effect of these medicinal plants on the gene expression of selected polyamine metabolic enzymes was determined using qPCR. Results: The IC50 ranged between 15 to 30 mg/ml for these three fruits. A549 growth indicated significant decreased in cell viability after 48 h. Annexin-V/PI demonstrated significant increase in early apoptosis in beetroot treated cells while other fruits caused significant increase in late apoptosis at 48 h of treatments. There was a significant downregulation of ODC gene in cells treated with jujube and raisins while upregulation of SSAT gene in beetroot treated cells. Conclusion: These medicinal fruits are the promising candidates for food-based chemoprevention strategy for their low polyamine content, vital in modulating polyamine metabolism and promoting cell death.