Expression of p40 immunohistochemistry in non-small cell lung carcinoma

Introduction: Lung cancer is the third most common cancers worldwide and in Malaysia. With the major advances in molecular testing of lung cancers and introduction of targeted therapies, the distinction between adenocarcinoma and squamous cell carcinoma (SCC) and its pathologic subtyping becomes im...

Full description

Saved in:
Bibliographic Details
Main Authors: Ahmad Affandi, Khairunisa, Md Zin, Reena Rahayu, Chandramaya, Sabrina, Mustangin, Muaatamarulain
Format: Article
Language:English
Published: College of Pathologists, Academy of Medicine Malaysia 2017
Subjects:
Online Access:http://irep.iium.edu.my/85947/1/abstracts%20iapmd%202017.pdf
http://irep.iium.edu.my/85947/
http://www.mjpath.org.my/2017/v39n3/abstracts.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
Institution: Universiti Islam Antarabangsa Malaysia
Language: English
id my.iium.irep.85947
record_format dspace
spelling my.iium.irep.859472020-12-14T01:20:07Z http://irep.iium.edu.my/85947/ Expression of p40 immunohistochemistry in non-small cell lung carcinoma Ahmad Affandi, Khairunisa Md Zin, Reena Rahayu Chandramaya, Sabrina Mustangin, Muaatamarulain RB Pathology Introduction: Lung cancer is the third most common cancers worldwide and in Malaysia. With the major advances in molecular testing of lung cancers and introduction of targeted therapies, the distinction between adenocarcinoma and squamous cell carcinoma (SCC) and its pathologic subtyping becomes important. Recent studies showed that p40 is highly specific for squamous and basal cells, and superior to p63 for the diagnosis of lung SCC. This study aimed to evaluate the use of p40 immunohistochemistry in diagnosis of non-small cell lung carcinoma and its potential to replace current p63 antibody as the best immunohistochemical squamous marker. Methods: Seventy formalin-fixed paraffin-embedded cases previously diagnosed primary lung SCC (n=35) and lung adenocarcinoma (n=35) from January 2008 to December 2016 were retrieved from Department of Pathology, Universiti Kebangsaan Malaysia. We compared the results of tumour cells immunoreactivity for p40 and p63 antibodies in lung SCC and lung adenocarcinoma. Results: The p40 was positive in 29 cases of previously diagnosed lung SCC (82.8%) and two cases of lung adenocarcinoma (5.7%). The p63 was positive in 31 lung SCC (88.6%) and 22 of lung adenocarcinoma (62.9%). The reactivity for both p40 and p63 in lung SCC was strong and diffuse whereas the reactivity of both antibodies in lung adenocarcinoma is variable. Conclusions: We found that the expression of p40 is equivalent to p63 in lung SCC, but p40 is an excellent marker in distinguishing lung SCC from adenocarcinoma. We suggest that p40 is considered for routine use and replace p63 as lung SCC marker. College of Pathologists, Academy of Medicine Malaysia 2017-12 Article NonPeerReviewed application/pdf en http://irep.iium.edu.my/85947/1/abstracts%20iapmd%202017.pdf Ahmad Affandi, Khairunisa and Md Zin, Reena Rahayu and Chandramaya, Sabrina and Mustangin, Muaatamarulain (2017) Expression of p40 immunohistochemistry in non-small cell lung carcinoma. The Malaysian Journal of Pathology, 39 (3). pp. 331-349. ISSN 0126-8635 http://www.mjpath.org.my/2017/v39n3/abstracts.pdf
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
topic RB Pathology
spellingShingle RB Pathology
Ahmad Affandi, Khairunisa
Md Zin, Reena Rahayu
Chandramaya, Sabrina
Mustangin, Muaatamarulain
Expression of p40 immunohistochemistry in non-small cell lung carcinoma
description Introduction: Lung cancer is the third most common cancers worldwide and in Malaysia. With the major advances in molecular testing of lung cancers and introduction of targeted therapies, the distinction between adenocarcinoma and squamous cell carcinoma (SCC) and its pathologic subtyping becomes important. Recent studies showed that p40 is highly specific for squamous and basal cells, and superior to p63 for the diagnosis of lung SCC. This study aimed to evaluate the use of p40 immunohistochemistry in diagnosis of non-small cell lung carcinoma and its potential to replace current p63 antibody as the best immunohistochemical squamous marker. Methods: Seventy formalin-fixed paraffin-embedded cases previously diagnosed primary lung SCC (n=35) and lung adenocarcinoma (n=35) from January 2008 to December 2016 were retrieved from Department of Pathology, Universiti Kebangsaan Malaysia. We compared the results of tumour cells immunoreactivity for p40 and p63 antibodies in lung SCC and lung adenocarcinoma. Results: The p40 was positive in 29 cases of previously diagnosed lung SCC (82.8%) and two cases of lung adenocarcinoma (5.7%). The p63 was positive in 31 lung SCC (88.6%) and 22 of lung adenocarcinoma (62.9%). The reactivity for both p40 and p63 in lung SCC was strong and diffuse whereas the reactivity of both antibodies in lung adenocarcinoma is variable. Conclusions: We found that the expression of p40 is equivalent to p63 in lung SCC, but p40 is an excellent marker in distinguishing lung SCC from adenocarcinoma. We suggest that p40 is considered for routine use and replace p63 as lung SCC marker.
format Article
author Ahmad Affandi, Khairunisa
Md Zin, Reena Rahayu
Chandramaya, Sabrina
Mustangin, Muaatamarulain
author_facet Ahmad Affandi, Khairunisa
Md Zin, Reena Rahayu
Chandramaya, Sabrina
Mustangin, Muaatamarulain
author_sort Ahmad Affandi, Khairunisa
title Expression of p40 immunohistochemistry in non-small cell lung carcinoma
title_short Expression of p40 immunohistochemistry in non-small cell lung carcinoma
title_full Expression of p40 immunohistochemistry in non-small cell lung carcinoma
title_fullStr Expression of p40 immunohistochemistry in non-small cell lung carcinoma
title_full_unstemmed Expression of p40 immunohistochemistry in non-small cell lung carcinoma
title_sort expression of p40 immunohistochemistry in non-small cell lung carcinoma
publisher College of Pathologists, Academy of Medicine Malaysia
publishDate 2017
url http://irep.iium.edu.my/85947/1/abstracts%20iapmd%202017.pdf
http://irep.iium.edu.my/85947/
http://www.mjpath.org.my/2017/v39n3/abstracts.pdf
_version_ 1687393122190884864