Biomass enhancement of Stevia Rebaudiana Bertoni shoot culture in temporary immersion system (TIS) RITA® bioreactor optimized in two different immersion periods
Stevia plant contains steviol glycosides, which are estimated to be 300 times sweeter than sucrose. However, conventional (in vivo) propagation of Stevia rebaudiana in Indonesia was not effective due to poor results. Therefore, an alternative method to propagate stevia plants is needed. One of t...
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| Main Authors: | , , , |
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| Format: | Conference or Workshop Item |
| Language: | English English |
| Published: |
EDP Sciences
2021
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| Subjects: | |
| Online Access: | http://irep.iium.edu.my/87652/7/87652_Biomass%20enhancement%20of%20Stevia.pdf http://irep.iium.edu.my/87652/13/87652_Biomass%20enhancement%20of%20stevia_scopus.pdf http://irep.iium.edu.my/87652/ https://www.e3s-conferences.org/articles/e3sconf/abs/2021/02/e3sconf_icon-beat2019_00007/e3sconf_icon-beat2019_00007.html |
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| Institution: | Universiti Islam Antarabangsa Malaysia |
| Language: | English English |
| Summary: | Stevia plant contains steviol glycosides, which are estimated to
be 300 times sweeter than sucrose. However, conventional (in vivo)
propagation of Stevia rebaudiana in Indonesia was not effective due to
poor results. Therefore, an alternative method to propagate stevia plants is
needed. One of them is by using in vitro method. Multiplication with a
large quantity of stevia biomass in a relatively short period can be
conducted by using TIS RITA® (Recipient for Automated Temporary
Immersion System). This study aimed to evaluate the effect of the
immersion period of the medium on growth and the medium bioconversion
into the production of shoot biomass. The bioreactors were set up with
15 min and 30 min immersions periods, scheduled every 6 h, and incubated
for 21 d. The result indicated that the immersion period affected the
biomass and growth rate (μ). Amount of 30 min immersion showed a
greater percentage of shoot multiplication, higher biomass, percentage of
leaf growth, growth rate, and productivity compared to 15 min immersion.
The pattern of sucrose, mineral, and inorganic compounds consumption
followed the growth of plant biomass for both systems. In conclusion,
30 min immersion gave a greater efficiency medium bioconversion to plant
biomass compared with 15 min immersion. |
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