A high mannose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: an in vitro investigation

Background: The primary cause of cancer is gene mutation which allows the growth of abnormal and damaged cells. Nutrition is one of the key factors that either increases or decreases the risk of cancer. Mannose has been found in many fruits such as oranges, apples and berries. Mannose has been lin...

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Main Authors: Mazlan, Muhammad Alif, Md Isa, Muhammad Lokman, Ibrahim, Moustafa
Format: Article
Language:English
English
Published: Springer Science and Business Media Deutschland GmbH 2020
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Online Access:http://irep.iium.edu.my/88258/1/88258_A%20high%20mannose%20concentration.pdf
http://irep.iium.edu.my/88258/2/88258_A%20high%20mannose%20concentration_SCOPUS.pdf
http://irep.iium.edu.my/88258/
https://jmhg.springeropen.com/track/pdf/10.1186/s43042-020-00109-w.pdf
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Institution: Universiti Islam Antarabangsa Malaysia
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spelling my.iium.irep.882582021-02-05T08:56:24Z http://irep.iium.edu.my/88258/ A high mannose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: an in vitro investigation Mazlan, Muhammad Alif Md Isa, Muhammad Lokman Ibrahim, Moustafa RC Internal medicine Background: The primary cause of cancer is gene mutation which allows the growth of abnormal and damaged cells. Nutrition is one of the key factors that either increases or decreases the risk of cancer. Mannose has been found in many fruits such as oranges, apples and berries. Mannose has been linked to increase the risk factors or potential therapeutic for cancers. However, insufficient information is available on the effects of high mannose concentration on the normal and cancer cell lines. This study aimed to evaluate the viability patterns of human cancer and normal cell lines treated with mannose. Human gingival fibroblast (HGF), skin malignant melanoma (A375) and colorectal adenocarcinoma (HT29) cell lines were cultured and treated with additional mannose in three respective concentrations: 1 mg/ml, 5 mg/ml and 10 mg/ml. Then, cell viability was measured using an MTT (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide)-assay. Results: The HGF cells’ percentage pattern of viability showed a rapid decline of nearly 95% on the third day of treatment. A375 cells were able to survive in high mannose condition as the cell viability percentage was at the highest value on Day 5. Meanwhile, HT29 cells showed declining cell viability pattern when treated with mannose. The data exhibited significance; the p value was less than 0.001. Conclusions: High mannose concentration can be toxic to HGF. In addition, A375 is adaptive to mannose at all concentrations in which it shares the same pattern with the untreated group. However, the cell viability pattern for HT29 cell is declining Springer Science and Business Media Deutschland GmbH 2020-12 Article PeerReviewed application/pdf en http://irep.iium.edu.my/88258/1/88258_A%20high%20mannose%20concentration.pdf application/pdf en http://irep.iium.edu.my/88258/2/88258_A%20high%20mannose%20concentration_SCOPUS.pdf Mazlan, Muhammad Alif and Md Isa, Muhammad Lokman and Ibrahim, Moustafa (2020) A high mannose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: an in vitro investigation. Egyptian Journal of Medical Human Genetics, 21 (1). ISSN 11108630 https://jmhg.springeropen.com/track/pdf/10.1186/s43042-020-00109-w.pdf 10.1186/s43042-020-00109-w
institution Universiti Islam Antarabangsa Malaysia
building IIUM Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider International Islamic University Malaysia
content_source IIUM Repository (IREP)
url_provider http://irep.iium.edu.my/
language English
English
topic RC Internal medicine
spellingShingle RC Internal medicine
Mazlan, Muhammad Alif
Md Isa, Muhammad Lokman
Ibrahim, Moustafa
A high mannose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: an in vitro investigation
description Background: The primary cause of cancer is gene mutation which allows the growth of abnormal and damaged cells. Nutrition is one of the key factors that either increases or decreases the risk of cancer. Mannose has been found in many fruits such as oranges, apples and berries. Mannose has been linked to increase the risk factors or potential therapeutic for cancers. However, insufficient information is available on the effects of high mannose concentration on the normal and cancer cell lines. This study aimed to evaluate the viability patterns of human cancer and normal cell lines treated with mannose. Human gingival fibroblast (HGF), skin malignant melanoma (A375) and colorectal adenocarcinoma (HT29) cell lines were cultured and treated with additional mannose in three respective concentrations: 1 mg/ml, 5 mg/ml and 10 mg/ml. Then, cell viability was measured using an MTT (3-(4,5- dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide)-assay. Results: The HGF cells’ percentage pattern of viability showed a rapid decline of nearly 95% on the third day of treatment. A375 cells were able to survive in high mannose condition as the cell viability percentage was at the highest value on Day 5. Meanwhile, HT29 cells showed declining cell viability pattern when treated with mannose. The data exhibited significance; the p value was less than 0.001. Conclusions: High mannose concentration can be toxic to HGF. In addition, A375 is adaptive to mannose at all concentrations in which it shares the same pattern with the untreated group. However, the cell viability pattern for HT29 cell is declining
format Article
author Mazlan, Muhammad Alif
Md Isa, Muhammad Lokman
Ibrahim, Moustafa
author_facet Mazlan, Muhammad Alif
Md Isa, Muhammad Lokman
Ibrahim, Moustafa
author_sort Mazlan, Muhammad Alif
title A high mannose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: an in vitro investigation
title_short A high mannose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: an in vitro investigation
title_full A high mannose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: an in vitro investigation
title_fullStr A high mannose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: an in vitro investigation
title_full_unstemmed A high mannose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: an in vitro investigation
title_sort high mannose concentration is well tolerated by colorectal adenocarcinoma and melanoma cells but toxic to normal human gingival fibroblast: an in vitro investigation
publisher Springer Science and Business Media Deutschland GmbH
publishDate 2020
url http://irep.iium.edu.my/88258/1/88258_A%20high%20mannose%20concentration.pdf
http://irep.iium.edu.my/88258/2/88258_A%20high%20mannose%20concentration_SCOPUS.pdf
http://irep.iium.edu.my/88258/
https://jmhg.springeropen.com/track/pdf/10.1186/s43042-020-00109-w.pdf
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