Optimization of modified-media containing serum for better rin-5f and brin-bd11 cells monolayer attachment, growth and insulin secretion

• Main Author: Abdul Karim, Mohamed Ismail
• Format: Conference or Workshop Item
• Language: English
• Published: 2009
• Subjects: TP248.13 Biotechnology
• Online Access: http://irep.iium.edu.my/8909/1/Seminar_on_Research_Findings_Series_No_2__2009__-_Prof_Ismail.pdf; http://irep.iium.edu.my/8909/
• Institution: Universiti Islam Antarabangsa Malaysia

A study was undertaken to observe the effect of D-glucose and L-glutamine and the combination of both nutrients at various concentrations towards the growth and insulin production by BRIN-BD11 cells. It was found that at a final concentration of 4 g/L D-glucose and 0.3 g/L L-glutamine in RPMI-1640 media gave maximum cell growth and insulin production of 1.75 x 106 cells/ml from 2.5 x 105 cells/ml and 100.77 µg/L, respectively. The result of this study indicates that D-glucose and L-glutamine promote good cell growth and insulin secretion of BRIN-BD11 cells as a step towards mass propagation of transplantable insulinoma cells and insulin production.