In silico CHARACTERIZATION OF UGT76G1 PROTEIN IN Stevia rebaudiana BERTONI ACCESSIONS MS007

• Main Authors: Samsulrizal, Nurul Hidayah, Zainuddin, Zarina, Meenakshi Sundram, Tamil Chelvan
• Format: Conference or Workshop Item
• Language: English; English; English; English; English
• Published: 2021
• Subjects: S Agriculture (General)
• Online Access: http://irep.iium.edu.my/90114/1/ABSTRACT-ICMBB.pdf; http://irep.iium.edu.my/90114/2/Poster%20Nor%20Iswani%20Mokthar.jpg; http://irep.iium.edu.my/90114/3/watch_v%3DSet056Ncdqk%26ab_channel%3D4thICMBB2021; http://irep.iium.edu.my/90114/13/Programme%20Book_4thICMBB2021%20%281%29.pdf; http://irep.iium.edu.my/90114/14/ICMBB2021%20Participation%20eCert.pdf; http://irep.iium.edu.my/90114/
• Institution: Universiti Islam Antarabangsa Malaysia

Stevia rebaudiana is a member of the Asteraceae family which is being consumed as an artificial sweetener. Stevia has been identified to be safe for human consumption and has been used as an alternative sweetener for diabetic and obese patients. This study used an in silico analysis of the transcriptome dataset of Stevia rebaudiana accession MS007 to describe the structure and gene content involved in the production of UGT76G1 protein. Homologous search using BLASTp resulting high similarity to UDP-glycosyltransferase 76G1-like Helianthus annuus (ID: XP_021973845.1) as the highest percentage of identity. Protein family search using InterPro showed the presence and entry of IPR002213 available at positions 89 to 246. The phylogenetic tree was constructed by selecting 14 out of 100 protein sequences from BLASTp using MEGA X software. The phylogenetic tree analysis showed the same protein family which is Asteraceae. The protein structure prediction of primary, secondary and tertiary of UGT76G1 protein was computed using ProtParam ExPasy, PSIPRED and Phyre2. The tertiary structure prediction of UGT76G1 protein scored 100.0% confidence by the single highest scoring template and coverage of 98% and the dimension of the model is (Å) of X: 52.453, Y: 61.270, and Z: 48.102. The findings of this study will lead to a deeper understanding of the characteristics of UDP-glycosyltransferase 76G1 (S. rebaudiana MS007) and enhance target recognition mechanisms, resulting in a better understanding of protein-protein interaction in Stevia rebaudiana MS007.