Immobilization of alpha-naphthyl acetate esterase (ANAE) on k-carrageenan for potential biosensor application in pesticide detection

• Main Authors: Mohamed Nageib, Adilah, Jameel, Ahmad Tariq, Ahmad, Farah
• Format: Article
• Language: English
• Published: Elsevier Ltd 2021
• Subjects: QC Physics; QD Chemistry; TP Chemical technology
• Online Access: http://irep.iium.edu.my/95097/7/95097_Immobilization%20of%20alpha-naphthyl%20acetate%20esterase.pdf; http://irep.iium.edu.my/95097/; https://www.sciencedirect.com/science/article/pii/S2214785321076240; https://doi.org/10.1016/j.matpr.2021.11.601
• Institution: Universiti Islam Antarabangsa Malaysia

In this study, alpha-naphthyl acetate esterase (ANAE) extracted from Atta (wholemeal) wheat flour and grinded wheat grains was immobilized on k-carrageenan beads that were amino-functionalized using 2% polyethyleneimine (PEI). ANAE is a plant esterase enzyme extracted from wheat, where its activity...