Isolation and characterization of beneficial endophytic bacteria from Oryza Sativa cultivated in acid sulfate soil

• Main Authors: Ponari, Md Hoirul Azri, A.Rani, Nor Adillah, Yunus, Muhamad Fahmi
• Format: Conference or Workshop Item
• Language: English; English; English
• Published: 2022
• Subjects: QR Microbiology
• Online Access: http://irep.iium.edu.my/99798/1/MSMPS2022%20Certificate%20%28Poster%20presentation_NOR%20ADILAH%20A.RANI%29.pdf; http://irep.iium.edu.my/99798/8/MSMPS%20Programme%20Book%202022.pdf; http://irep.iium.edu.my/99798/20/99798_Isolation%20and%20characterization%20of%20beneficial%20endophytic.pdf; http://irep.iium.edu.my/99798/
• Institution: Universiti Islam Antarabangsa Malaysia

Abstract: Pyrite (FeS2) oxidation in acid sulfate soil led to high acidity and aluminium (Al) and/or iron (Fe) toxicity to the plant. This problem causes nutrient deficiency and root inhibition, which retards plant nutrient uptake efficiency for growth. Plant-microbe interaction is one of the most important determining factors influencing nutrient uptake and plant growth in acid sulfate soil. Thus, this research aims to isolate and characterize the beneficial endophytic bacteria from Oryza sativa cultivated in acid sulfate soil. The O. sativa samples were collected from a paddy field in Nenasi, Pekan, Pahang. Eight endophytic bacteria were successfully isolated using serial dilution methods after surface sterilization of plant samples was achieved. All isolated bacteria showed different capacities for plant growth-promotion features, including production of plant growth hormone, solubilization of phosphate and converting atmospheric nitrogen into a plant-usable form. This preliminary analysis found that the isolated bacteria have potential for use in integrated plant nutrient management that promotes vegetation growth in acid sulfate soils. Keywords: plant growth-promoting bacteria, indole 3-acetic acid, phosphate solubilization, biological nitrogen fixation.